Bovine lipopolysaccharide (LPS) ELISA kit is an enzyme-linked immunosorbent assay for the quantitative detection of bovine LPS in samples and its detection range is 50-100 µL.
Assay Principle
Bovine lipopolysaccharide (LPS) ELISA kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique. Monoclonal antibodies against bovine LBP are precoated on the microplate. After the sample and biotinylated antibodies are added to the plate and allowed to react, the plate is washed with PBS or TBS. Then, a peroxidase-conjugated avidin is added and allowed to react, followed by thorough washing with PBS or TBS. Subsequently, a TMB substrate is added for color development. TMB turns blue in the presence of the peroxidase enzyme and is converted to the final yellow color under acidic conditions. The intensity of the color is directly proportional to the concentration of the target factor in the sample.
Serum, plasma, cell culture supernatant and tissue samples
Detection Method
Colorimetric, OD 450 nm
Assay Range
50-100 µL
Precision (Intra-Assay)
CV <8%
Precision (Inter-Assay)
CV <10%
Recovery
85-110%
Cross-reactivity
No significant cross-reactivity or interference was observed.
Applications
Bovine lipopolysaccharide (LPS) ELISA kit can be used to measure the level of bovine LPS in serum, plasma, cell culture supernatant and tissue samples.
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