Bovine lipopolysaccharide (LPS) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of bovine LPS as low as 1 EU/L and its detection range is 25-800 EU/L.
Assay Principle
Bovine lipopolysaccharide (LPS) ELISA kit is used to measure the level of bovine LPS in a sample. Initially, purified bovine LPS antibody is used to coat the wells of a microtiter plate, creating a solid-phase antibody. Next, LPS is added to the wells, and the antibody, which is labeled with HRP, binds to the LPS, forming an antibody-antigen-enzyme-antibody complex. After thorough washing, a TMB substrate solution is added, resulting in the substrate turning blue in the presence of the HRP enzyme. The reaction is then stopped by adding a sulfuric acid solution, and the color change is measured at a wavelength of 450 nm using a spectrophotometer. The concentration of LPS in the samples is determined by comparing the optical density (O.D.) of the samples to a standard curve.
Serum, plasma, tissue homogenates and other biological fluids
Detection Method
Colorimetric, OD 450 nm
Assay Range
25-800 EU/L
Sensitivity
1 EU/L
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Recovery
>95%
Cross-reactivity
No significant cross-reactivity or interference was observed.
Applications
Bovine lipopolysaccharide (LPS) ELISA kit can be used to measure the level of bovine LPS in serum, plasma, tissue homogenate and other biological fluid samples.
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