Lacto-N-neotetraose (LNnT) is expected to be added to infant food as a nutritional fortifier. Exploring its production methods of it and its derivatives has become a major research hotspot. CD BioGlyco presents multiple strategies to achieve efficient production of LNnT.
Human milk oligosaccharides (HMOs) can be divided into three types of oligosaccharides, which are neutral oligosaccharides, fucosylated oligosaccharides, and sialylated oligosaccharides. As one of the important core structures of HMOs, LNnT is the simplest type II sugar, consisting of galactose (Gal), N-acetylglucosamine (GlcNAc), and glucose (Glc), the structure is shown in Figure 1. It can be synthesized into various derivatives through sialylation and fucosylation, including lacto-N-fucopentaose, lacto-N-neofucopentaose, lacto-N-neodifuco-hexaose, and sialyllacto-N-tetraose. The derivatives have unique effects on the human body, which have been confirmed by many studies. In short, LNnT and its derivatives have great application potential and commercial value in the food and pharmaceutical industries. The exploration of related production methods has become a major research hotspot.
LNnT has a prebiotic effect and can promote the growth and metabolic activities of bifidobacteria in the intestine, which can significantly increase the number and population abundance of the strains.
For example, LNnT and its derivatives can stimulate type II immunity by increasing the number of Grl cells and antigen-presenting cells.
LNnT and its sialylated derivatives can block the adhesion of Streptococcus pneumonia to intestinal epithelial cells, thus inhibiting the occurrence of pneumonia.
We employ highly specific glycosyltransferases, precisely control the sequence and linkage of the sugar units, ensuring the final product is identical to the LNnT found in human milk.
To achieve scalable and cost-effective production, we integrate enzymatic synthesis with state-of-the-art metabolic engineering. By engineering these microbial hosts, we optimize their metabolic pathways to not only produce the necessary enzymes for synthesis but also to generate the required sugar precursors.
CD BioGlyco provides a variety of Methods to produce LNnT and its derivatives. Due to the specificity of the enzymatic reaction, the latter two synthetic methods are the most efficient and promising, and progress has been especially great in developing and improving these two methods through the continuous efforts of our researchers.
Our production strategy includes but is not limited to the following:
Journal: Scientific reports
IF: 3.8
Published: 2020
Results: This study examines LNnT for its effects on full-thickness skin wound healing and type 2 immune response-related gene expression. In vitro, the MTT assay confirmed that LNnT did not impair normal human dermal fibroblast viability. In vivo, the results showed LNnT accelerated wound closure; histological analyses revealed better healing scores, more hair follicles, normal-like epidermal thickness, higher collagen deposition, and lower collagen; stereology found fewer neutrophils, more fibroblasts (early stage) and basal cells; real-time polymerase chain reaction (PCR) upregulated IL-4, IL-10 (anti-inflammatory) and IL-13. Conclusion: LNnT promotes wound healing via anti-inflammatory properties and type 2 immune induction, making it a promising wound care candidate.
Unrivaled Purity
Our sophisticated enzymatic synthesis and multi-stage purification protocols yield LNnT with exceptional purity, minimizing impurities that could affect product performance or safety.
Scalability and Consistency
We possess the infrastructure and expertise to scale LNnT production from laboratory to industrial quantities, ensuring consistent quality and reliable supply to meet growing market demands.
Expertise in Glycoscience
With over years of experience, CD BioGlyco's team comprises world-class experts in glycobiology and synthetic chemistry, bringing unparalleled knowledge to every project.
Customization Flexibility
We offer flexible service options, including custom synthesis parameters and purity specifications, to perfectly align with specific project requirements.
Functional Food and Beverage Development
LNnT can be used to develop a wide range of products, such as yogurts, juices, and nutritional supplements, to enhance their prebiotic properties.
Dietary Supplements for Gut and Immune Health
The immune-modulatory and prebiotic effects of LNnT make it a perfect active ingredient for dietary supplements aimed at gut health and immune support.
Fundamental Biomedical Research
Researchers use high-purity LNnT to study its role in gut-brain axis communication, immune system regulation, and its potential in treating various diseases.
"CD BioGlyco's technical team provided us with a truly exceptional level of service for our bulk LNnT order. The purity levels exceeded our internal QC standards, and their ability to scale from a pilot batch to commercial tonnage was rapid. We view them as a key, long-term strategic supplier."
- P.A., Nutrition Provider
"We were previously struggling with inconsistent purity from another supplier's chemical synthesis route. Switching to CD BioGlyco's bio-fermentation LNnT solved our isomer issue completely. The consistency across batches is flawless, which is essential for our precision pharmaceutical research. Their process validation reports were detailed and transparent, reflecting true scientific integrity."
- Dr. S.K., Biotech Company
"The flexibility in ordering research-grade material for our assay development, followed by a smooth transition to bulk production, was seamless. The personalized support from our account manager and the production team made a complex project straightforward."
- J.D., Dietary Supplement Firm
As a recognized expert in the production of LNnT, CD BioGlyco has developed efficient and high-quality synthetic strategies. If you have any needs in this regard, please feel free to contact us.
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