Properties
Description
Microorganism lipopolysaccharide-antibody (LPS-Ab) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of microorganism LPS-Ab as low as 1.0 EU/L and its detection range is 25-800 EU/L.
Assay Principle
The kit employs the indirect enzyme-linked immunosorbent assay (ELISA) method. Microtiter plate wells are pre-coated with antibodies specific to microbial lipopolysaccharide (LPS-Ab). The assay is performed by sequentially adding the sample, standard, and HRP-labeled detection antibodies to the wells. After incubation and thorough washing, a substrate called TMB is added, which undergoes a color change to blue in the presence of the peroxidase enzyme. Upon addition of an acidic solution, the color turns into the final yellow form. The intensity of the color is directly proportional to the presence of microbial lipopolysaccharide antibodies (LPS-Ab) in the sample. The absorbance (OD value) is measured at a wavelength of 450nm using a microplate reader, and the sample concentration is calculated based on this measurement.
Species Reactivity
Microorganism
Components
MicroELISA stripplate; Standard; Sample diluent; Enzyme conjugate; Wash solution; Chromogen solution A; Chromogen solution B; Stop solution; Closure plate membrane; Sealed bags
Sample Type
Biological fluids
Detection Method
Colorimetric, OD 450 nm
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Applications
Microorganism lipopolysaccharide-antibody (LPS-Ab) ELISA kit can be used to measure the level of microorganism LPS-Ab in biological fluid samples.
