Mouse lipopolysaccharide-binding protein (LBP) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of mouse LPS-Ab as low as 0.1 ng/mL and its detection range is 0.5-16 ng/mL.
Assay Principle
Mouse lipopolysaccharide antibody (LPS-Ab) ELISA kit is designed to measure the level of Mouse LPS antibodies (LPS-Ab) in a sample. It involves coating the wells of a microtiter plate with purified Mouse LPS antibody antigen, creating a solid-phase antigen. The sample is then added to the wells, and the LPS antibodies present in the sample bind to the immobilized antigen, forming an antigen-antibody complex. HRP-labeled antibodies that specifically recognize the Mouse LPS antibodies are added, resulting in the formation of an antigen-antibody-enzyme-antibody complex. After thorough washing to remove any unbound components, a TMB substrate solution is added. In the presence of the enzyme HRP, the TMB substrate undergoes a color change, turning blue. The reaction is stopped by adding a sulfuric acid solution, and the resulting color change is measured using a spectrophotometer at a wavelength of 450 nm. By comparing the optical density (O.D.) of the samples to a standard curve, the concentration of Mouse LPS antibodies in the samples can be determined.
Serum, plasma, tissue homogenates and other biological fluids
Detection Method
Colorimetric, OD 450 nm
Assay Range
0.5-16 ng/mL
Sensitivity
0.1 ng/mL
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Recovery
>95%
Applications
Mouse lipopolysaccharide-binding protein (LBP) ELISA kit can be used to measure the level of mouse LBP in serum, plasma, cell culture supernatant and tissue samples.
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