Tumor-Associated Le^a Antigen Production Service at CD BioGlyco

• The functions of Le^a antigen in biology

The Le^a antigen, including its sialylated form sLe^a, is a blood group antigen existing in glycolipids and glycoproteins on cell surfaces, including erythrocytes and various normal cells. While sLe^a is naturally present in specific normal tissues such as fibroblasts and epithelial cells, it is significantly elevated in several cancer types marked by increased fucosylation. These cancers encompass colon, pancreatic, gallbladder/bile duct, breast, cholangiocarcinoma, and small-cell lung cancer. Recognized as CA19-9, sLe^a is an early tumor-associated carbohydrate antigen (TACA) and serves as a valuable serum biomarker for prognosis, postoperative monitoring, and assessing therapeutic responses in pancreatic and gastrointestinal cancers.

• Two Le^a antigen production approaches at CD BioGlyco

The intricate structural complexity of Lewis blood group antigens has intrigued numerous synthetic carbohydrate researchers. The sulfoxide glycosylation method offers a uniform set of reaction conditions that enable the stereo-selective formation of all glycosidic linkages within the Lewis blood group antigens Le^a, Le^b, and Le^x, as illustrated in Fig.1. A gram-scale synthesis of Le^a antigens (Le^aProN₃) and a library of sialyl Le^a antigens (sLe^aProN₃) including various sialic acid forms may also be produced using a chemoenzymatic technique (Fig.2).

Fig.1 The synthesis of Le^a began with the coupling of phenyl 2-azido-4,6-O-benzylidene-2-deoxy-1-thio-R-D-glucopyranoside with the per-pivaloyl galactosyl sulfoxide. (Yan & Kahne, 1996)

Fig.2 The chemoenzymatic approach for the gram-scale synthesis of Le^a. (Tasnima, et al., 2019)

• Our custom Le^a antigen production solution

By combining existing synthetic routes and literature support, our company can customize synthetic routes for different product sizes, purities, and application scenarios. Here is a standard of our company's process of customized solutions for production services. Relying on our advanced experimental platform and excellent experimental staff, as well as the support of our chemists, we are confident that we can be the right hand of our clients in the field of sugar chemistry, providing our support. Besides, we also provide other Tumor-Associated Lacto-series Antigen Production Services such as SLe^a Antigen and Le^b Antigen, and we expect to work for clients in the field of Tumor-Associated Carbohydrate Vaccine Development.

Fig.3 The standard scheme for the synthesis of Le^a antigen. (CD BioGlyco)

Elevated Le^a antigen levels in blood serve as an indicator of cancer presence, aiding early diagnosis. It plays a pivotal role in determining prognosis, postoperative monitoring, and assessing therapeutic responses. Monitoring Le^a antigen levels during cancer treatment provides valuable insights. Additionally, Le^a antigen contributes to research on aberrant glycosylation patterns in cancer cells, shedding light on cancer biology and potential targeted therapies.

Applications of Tumor-Associated Le^a Antigen Production Service

• Tumor-associated antigens like Le^a are pivotal in the quest for cancer biomarkers. Le^a antigens can be harnessed to create diagnostic assays for a range of cancers, including pancreatic, colorectal, and lung cancer.
• The Le^a antigen serves as a valuable biomarker for the early detection of cancer, enabling prompt intervention and treatment.
• Le^a antigen becomes a vital target in immunotherapy endeavors. It underpins strategies like cancer vaccines and antibody-based therapies, paving the way for innovative cancer treatments.
• Le^a antigen is a critical tool in drug development investigations. Researchers can utilize it to assess the effectiveness of novel cancer treatments and therapies.
• In the realm of biological research, the Le^a antigen sheds light on the role of carbohydrate antigens in cancer biology and the intricate mechanisms that drive cancer progression.

Advantages of Us

• We offer custom production and analysis services for clients according to their requirements and budgets.
• Our service team is available to respond to inquiries from clients worldwide.
• We provide different scales of Le^a antigen products from mg level to g level.

CD BioGlyco has established a well-equipment lab to provide our clients with strong support for Le^a antigen production and professional custom services. If you would like to know more about our service, please do not hesitate to contact us.

References

1. Yan, L.; Kahne, D. Generalizing glycosylation: synthesis of the blood group antigens Le^a, Le^b, and Le^x using a standard set of reaction conditions. Journal of the American Chemical Society. 1996, 118(39): 9239-9248.
2. Tasnima, N.; et al. Facile chemoenzymatic synthesis of Lewis a (Le^a) antigen in gram-scale and sialyl Lewis a (sLe^a) antigens containing diverse sialic acid forms. Carbohydrate Research. 2019, 472: 115-121.