Overview

Anomalous intercellular sugar chains have been linked to numerous diseases, including cancer and rheumatism. The development of highly sensitive detection methods for sugar chains is therefore essential for advancing biological and pathological studies. CD BioGlyco offers a state-of-the-art high-performance liquid chromatography with charged aerosol detection (HPLC-CAD) based service for the direct and highly sensitive analysis of oligosaccharides. This novel strategy overcomes traditional limitations, delivering a robust and reliable solution across diverse R&D applications.

Key Technologies

HPLC-CAD is a sophisticated detection method that operates on the principle of nebulizing the column eluent with nitrogen gas, evaporating the mobile phase, and then charging the resulting dried particles by impacting them with positively charged nitrogen cations. The amount of electric charge on the eluent is then measured, providing a universal detection mechanism for compounds, even those lacking chromophores or ultraviolet (UV) absorbance.

Oligosaccharides Unlocked: HPLC-CAD Precision Revealed

We offer comprehensive capabilities for the detection and quantification of various oligosaccharides. Our service is particularly adept at the direct detection of underivatized oligosaccharides, eliminating the need for time-consuming and complicated fluorescent derivatization steps often associated with traditional methods. We are equipped to analyze sialylglycopeptides (SGPs) and their hydrolysis products, including monosialo- and asialo-oligosaccharides, as well as free sialic acid. Our capabilities extend to comparing detection sensitivities across different methods (CAD, UV absorbance, and fluorescence detection). The versatility of our HILIC-CAD system, employing amido-silica columns, allows for the effective separation and analysis of a wide range of oligosaccharides. This service is ideal for projects requiring highly sensitive detection and characterization of sugar chains without the drawbacks of conventional derivatization techniques.

Workflow

Our analysis service follows a streamlined and rigorous workflow to ensure accurate and reliable results:

Sample Reception and Assessment

Upon receipt, samples undergo initial quality assessment to confirm suitability for analysis. This includes evaluating sample integrity, concentration, and any specific client requirements.

Method Development and Optimization

Our expert team designs and optimizes the HPLC-CAD method specifically for your oligosaccharide samples. This involves selecting appropriate columns, mobile phases, and detection parameters to achieve optimal separation and sensitivity. Modifiers like trifluoroacetic acid (TFA) are carefully incorporated to improve peak shapes and enhance sensitivity by decreasing background current.

Sample Preparation and Injection

Samples are meticulously prepared according to the optimized method, including any necessary dilutions or filtrations. A fixed injection volume is used for consistent analysis.

HPLC-CAD Analysis

The prepared samples are then analyzed using our HPLC-CAD system. This step involves precise control of flow rates and column temperature to ensure robust separation and detection.

Data Acquisition and Processing

Chromatographic data, including peak areas and heights, are acquired and processed using advanced integration software. Calibration curves are constructed using authentic standards to ensure linearity and accurate quantification.

Interpretation and Reporting

Our scientists interpret the acquired data, identifying and quantifying oligosaccharides. This may include identifying hydrolysis products, such as monosialo- and asialo-oligosaccharides, and free sialic acid. A comprehensive report is then generated, detailing the findings.

Publication Data

Associated Services

Precision characterization of oligosaccharides via HPLC-CAD analysis is the essential first step toward understanding their structure and function. This rigorous analytical foundation now propels the next frontier: sophisticated library construction. Building upon the insights gained from HPLC-CAD profiling, we offer tailored synthesis solutions to generate bespoke oligosaccharide collections:

Applications

• Our analysis service is useful for identifying potential oligosaccharide biomarkers for disease diagnosis, prognosis, and monitoring.
• Our analysis service can be used to analyze oligosaccharides in food products, providing insights into their nutritional value, prebiotic properties, and potential health benefits.
• Our analysis service is applied to evaluate the success of glycan engineering efforts or to study the pathways and enzymes involved in oligosaccharide biosynthesis.

Advantages

• Our method enables the direct detection of oligosaccharides without requiring complex and time-consuming fluorescent derivatization.
• Our service allows for the detection of compounds that do not absorb UV light, offering a universal detection capability.
• Our strategy is highly effective for analyzing acid hydrolysis products of oligosaccharides, allowing for the detection of monosialo- and asialo-oligosaccharides, as well as free sialic acid.

Frequently Asked Questions

CD BioGlyco is committed to providing unparalleled scientific expertise and cutting-edge analytical solutions for your oligosaccharide analysis and Oligosaccharide Synthesis needs. Our HPLC-CAD-based service is a testament to our dedication to innovation and quality, offering a powerful tool for advancing your research. For detailed information, project consultations, or to request a quote, please do not hesitate to contact our expert team.