HPLC-CAD-based Oligosaccharides Analysis Service
Anomalous intercellular sugar chains have been linked to numerous diseases, including cancer and rheumatism. The development of highly sensitive detection methods for sugar chains is therefore essential for advancing biological and pathological studies. CD BioGlyco offers a state-of-the-art high-performance liquid chromatography with charged aerosol detection (HPLC-CAD) based service for the direct and highly sensitive analysis of oligosaccharides. This novel strategy overcomes traditional limitations, delivering a robust and reliable solution across diverse R&D applications.
HPLC-CAD is a sophisticated detection method that operates on the principle of nebulizing the column eluent with nitrogen gas, evaporating the mobile phase, and then charging the resulting dried particles by impacting them with positively charged nitrogen cations. The amount of electric charge on the eluent is then measured, providing a universal detection mechanism for compounds, even those lacking chromophores or ultraviolet (UV) absorbance.
We offer comprehensive capabilities for the detection and quantification of various oligosaccharides. Our service is particularly adept at the direct detection of underivatized oligosaccharides, eliminating the need for time-consuming and complicated fluorescent derivatization steps often associated with traditional methods. We are equipped to analyze sialylglycopeptides (SGPs) and their hydrolysis products, including monosialo- and asialo-oligosaccharides, as well as free sialic acid. Our capabilities extend to comparing detection sensitivities across different methods (CAD, UV absorbance, and fluorescence detection). The versatility of our HILIC-CAD system, employing amido-silica columns, allows for the effective separation and analysis of a wide range of oligosaccharides. This service is ideal for projects requiring highly sensitive detection and characterization of sugar chains without the drawbacks of conventional derivatization techniques.
Our analysis service follows a streamlined and rigorous workflow to ensure accurate and reliable results:
Upon receipt, samples undergo initial quality assessment to confirm suitability for analysis. This includes evaluating sample integrity, concentration, and any specific client requirements.
Our expert team designs and optimizes the HPLC-CAD method specifically for your oligosaccharide samples. This involves selecting appropriate columns, mobile phases, and detection parameters to achieve optimal separation and sensitivity. Modifiers like trifluoroacetic acid (TFA) are carefully incorporated to improve peak shapes and enhance sensitivity by decreasing background current.
Samples are meticulously prepared according to the optimized method, including any necessary dilutions or filtrations. A fixed injection volume is used for consistent analysis.
The prepared samples are then analyzed using our HPLC-CAD system. This step involves precise control of flow rates and column temperature to ensure robust separation and detection.
Chromatographic data, including peak areas and heights, are acquired and processed using advanced integration software. Calibration curves are constructed using authentic standards to ensure linearity and accurate quantification.
Our scientists interpret the acquired data, identifying and quantifying oligosaccharides. This may include identifying hydrolysis products, such as monosialo- and asialo-oligosaccharides, and free sialic acid. A comprehensive report is then generated, detailing the findings.
Journal: Scientific Reports
IF: 3.8
DOI: 10.1038/s41598-023-37832-4
Published: 2023
Results: This study developed a novel, universal UHPLC-CAD platform, termed UniQS, for directly quantifying and characterizing intact polysaccharide antigens used in glycoconjugate vaccines (e.g., against Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus). Recognizing the limitations of traditional methods that require specific hydrolysis to monosaccharides and tailored development for each PS serotype, the authors created two complementary ultra-high performance liquid chromatography (UHPLC) methods: a reversed-phase (RP) method (UniQS-1) for neutral and carboxylic acid-containing polysaccharides, and a hydrophilic interaction chromatography (HILIC) method (UniQS-2) specifically for charged/phosphodiester-containing polysaccharides. This platform enables the direct analysis of underivatized polysaccharides without complex derivatization or serotype-specific hydrolysis steps, allows quantification throughout the vaccine production process (from crude fermentation broth to purified polysaccharide), and demonstrates good linearity. The UniQS approach offers significant advantages in speed, cost, and applicability for developing multivalent vaccines by providing a universal analytical strategy for diverse polysaccharide antigens.
Precision characterization of oligosaccharides via HPLC-CAD analysis is the essential first step toward understanding their structure and function. This rigorous analytical foundation now propels the next frontier: sophisticated library construction. Building upon the insights gained from HPLC-CAD profiling, we offer tailored synthesis solutions to generate bespoke oligosaccharide collections:
| Modular Synthesis-based Oligosaccharide Library Construction Service | Enzymatic Synthesis-based Oligosaccharide Library Construction Service | Chemical Synthesis-based Oligosaccharide Library Construction Service |
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| For rapid, combinatorial assembly of diverse glycans using pre-validated building blocks. | Leveraging nature's precision for stereospecific, biologically relevant structures. | Enabling access to rare, non-natural analogs through advanced glycosylation strategies. |
CD BioGlyco is committed to providing unparalleled scientific expertise and cutting-edge analytical solutions for your oligosaccharide analysis and Oligosaccharide Synthesis needs. Our HPLC-CAD-based service is a testament to our dedication to innovation and quality, offering a powerful tool for advancing your research. For detailed information, project consultations, or to request a quote, please do not hesitate to expert team.
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