Nucleotide Enzymatic Digestion Service
Nucleotide sugars are foundational molecules in biology, serving as essential precursors for the biosynthesis of complex carbohydrates, glycolipids, and glycoproteins. These biomolecules play a critical role in cellular communication, immune response, and structural integrity. CD BioGlyco leverages advanced enzymatic digestion to provide a superior solution for the large-scale, high-purity production of these valuable compounds. This innovative approach overcomes the limitations of traditional chemical synthesis by offering enhanced specificity, higher yields, and a more sustainable process. By converting raw materials like RNA into high-value 5'-ribonucleotides such as 5'-IMP, 5'-GMP, 5'-CMP, 5'-UMP, and 5'-AMP, our service unlocks new possibilities for research and industrial applications across the food and pharmaceutical sectors.
Our production platform is built upon a sophisticated enzymatic process that utilizes highly specific enzymes to hydrolyze complex substrates with unparalleled precision. The cornerstone of this technology is the use of immobilized enzymes, which are enzymes covalently linked to a solid support, such as an acrylic resin. This immobilization method provides several significant advantages over using soluble enzymes, including enhanced stability, increased reusability, and the ability to operate in a continuous process.
At CD BioGlyco, we offer a variety of methods to Produce Nucleotides. Enzymatic hydrolysis is one of the more widely used methods in the industry. At CD BioGlyco, we use three main types of enzymes: ribonucleic acid hydrolases, deoxyribonucleic acid hydrolases, and ribonucleic acid hydrolases for DNA and RNA.
Based on this, we have conducted extensive research in the production of enzymes and offer both fermentation and plant root extraction methods for enzyme production.
For enzymatic production of nucleotides, CD BioGlyco offers the following pathways:
Fig.1 Types of enzymes and enzymatic hydrolysis. (CD BioGlyco)
We have designed a streamlined and scalable workflow to ensure the efficient production of nucleotide sugars. Our process is a continuous two-step system using packed-bed reactors, which allows for precise control and high throughput.
High-quality raw materials, such as yeast RNA, are prepared into a solution to be used as the primary substrate.
The prepared substrate is fed into the first reactor, which contains the immobilized 5'-phosphodiesterase. This reactor operates at a controlled temperature and pH to facilitate the efficient hydrolysis of RNA into a mixture of 5'-ribonucleotides.
The product from the first reactor is then transferred to a second reactor. This reactor, containing immobilized 5'-adenylate deaminase, operates at a different, lower temperature to perform the targeted deamination of 5'-AMP to 5'-IMP.
The product stream from the final reactor undergoes a multi-stage purification process to isolate the desired nucleotide sugars and remove any residual impurities.
DOI.: 10.1590/1981-6723.24620
Journal: Brazilian Journal of Food Technology
Published: 2021
Results: The authors explored a sustainable method for producing flavor-enhancing 5'-ribonucleotides (like IMP and GMP) using 5'-phosphodiesterase (5'-PDE) enzyme extracted from spent malt roots—a brewing industry byproduct. They aimed to valorize this waste material as an alternative, low-cost enzyme source. The study involved extracting and partially purifying 5'-PDE from the roots. The enzyme's activity was then tested for hydrolyzing RNA (from yeast/torula) under optimized conditions (pH, temperature, incubation time). Key outcomes included demonstrating the enzyme's effectiveness in releasing 5'-ribonucleotides and quantifying the yield of specific nucleotides (e.g., GMP, AMP, UMP, CMP). Results highlighted spent malt roots as a viable, economical source of 5'-PDE for nucleotide production, offering a sustainable alternative to commercial enzymes.
Following our nucleotide enzymatic digestion service to isolate specific nucleotide monomers, we offer further customization through specialized associated services. These enable precise modifications to the nucleoside's sugar moiety, including Sugar-based Modification Services such as halogenation, methylation, ring modification, saturation adjustment, hydroxylation/dehydroxylation, as well as glycosidic bond modification and stereo-/enantio-selective reactions—tailored to advance your functional or structural objectives.
CD BioGlyco has considerable experimental experience in producing nucleotides. We have advanced laboratory equipment and experienced researchers to provide custom services and guarantee the results of our clients' experiments.
Customers can contact our staff via email or call directly and we will reply promptly. If you are interested in our services, please feel free to contact us for more details.
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