Overview

Nucleotide sugars are foundational molecules in biology, serving as essential precursors for the biosynthesis of complex carbohydrates, glycolipids, and glycoproteins. These biomolecules play a critical role in cellular communication, immune response, and structural integrity. CD BioGlyco leverages advanced enzymatic digestion to provide a superior solution for the large-scale, high-purity production of these valuable compounds. This innovative approach overcomes the limitations of traditional chemical synthesis by offering enhanced specificity, higher yields, and a more sustainable process. By converting raw materials like RNA into high-value 5'-ribonucleotides such as 5'-IMP, 5'-GMP, 5'-CMP, 5'-UMP, and 5'-AMP, our service unlocks new possibilities for research and industrial applications across the food and pharmaceutical sectors.

Key Technologies

Our production platform is built upon a sophisticated enzymatic process that utilizes highly specific enzymes to hydrolyze complex substrates with unparalleled precision. The cornerstone of this technology is the use of immobilized enzymes, which are enzymes covalently linked to a solid support, such as an acrylic resin. This immobilization method provides several significant advantages over using soluble enzymes, including enhanced stability, increased reusability, and the ability to operate in a continuous process.

Nucleotide Enzymatic Digestion Service at CD BioGlyco

At CD BioGlyco, we offer a variety of methods to Produce Nucleotides. Enzymatic hydrolysis is one of the more widely used methods in the industry. At CD BioGlyco, we use three main types of enzymes: ribonucleic acid hydrolases, deoxyribonucleic acid hydrolases, and ribonucleic acid hydrolases for DNA and RNA.

Based on this, we have conducted extensive research in the production of enzymes and offer both fermentation and plant root extraction methods for enzyme production.

For enzymatic production of nucleotides, CD BioGlyco offers the following pathways:

• Direct hydrolysis of the enzyme solution
  This route greatly improves the enzyme activity and ends up with a 95% yield of nucleotides.
• Immobilized enzyme hydrolysis method
  In this method, the enzyme selectivity, thermal stability, and acid-base stability are all greatly improved.
• Enzyme-membrane reactor method
  We provide clients with this method which allows for the simultaneous completion of the reaction and separation of the products, helping clients to realize efficient, continuous production.

Fig.1 Types of enzymes and enzymatic hydrolysis. (CD BioGlyco)

Workflow

We have designed a streamlined and scalable workflow to ensure the efficient production of nucleotide sugars. Our process is a continuous two-step system using packed-bed reactors, which allows for precise control and high throughput.

• Substrate Preparation

High-quality raw materials, such as yeast RNA, are prepared into a solution to be used as the primary substrate.

• Primary Hydrolysis

The prepared substrate is fed into the first reactor, which contains the immobilized 5'-phosphodiesterase. This reactor operates at a controlled temperature and pH to facilitate the efficient hydrolysis of RNA into a mixture of 5'-ribonucleotides.

• Secondary Conversion (Deamination)

The product from the first reactor is then transferred to a second reactor. This reactor, containing immobilized 5'-adenylate deaminase, operates at a different, lower temperature to perform the targeted deamination of 5'-AMP to 5'-IMP.

• Purification

The product stream from the final reactor undergoes a multi-stage purification process to isolate the desired nucleotide sugars and remove any residual impurities.

Publication Data

Applications

• In the agricultural field, nucleotides are used in the research and development of feed.
• In the medical field, nucleotides could be used in research for the production of anti-tumor and anti-viral drugs.
• Nucleotides could be used in the production of food additives in infant milk powder.

Advantages

• Our highly specific enzymatic process and advanced purification techniques ensure a final product of exceptional purity and high yield.
• The use of immobilized enzymes provides superior thermal and operational stability, allowing for repeated use and a more cost-effective production process compared to traditional soluble enzymes.
• Our two-reactor continuous flow system enables higher productivity and greater control over the process, ensuring consistent product quality over extended operation times.

Associated Services

Following our nucleotide enzymatic digestion service to isolate specific nucleotide monomers, we offer further customization through specialized associated services. These enable precise modifications to the nucleoside's sugar moiety, including Sugar-based Modification Services such as halogenation, methylation, ring modification, saturation adjustment, hydroxylation/dehydroxylation, as well as glycosidic bond modification and stereo-/enantio-selective reactions—tailored to advance your functional or structural objectives.

Frequently Asked Questions

CD BioGlyco has considerable experimental experience in producing nucleotides. We have advanced laboratory equipment and experienced researchers to provide custom services and guarantee the results of our clients' experiments.

Customers can contact our staff via email or call directly and we will reply promptly. If you are interested in our services, please feel free to contact us for more details.

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