Recombinant Bacillus subtilis pectin lyase 1A produced by Escherichia coli, is responsible for plant cell wall decomposition by cleaving pectin through a β-elimination mechanism. It is produced in Escherichia coli.
Expression System
Escherichia coli
Species
Bacillus subtilis
Concentration
0.25 mg/mL
Form
Liquid
Purity
≥90%, determined by SDS-PAGE.
Activity
Pectin lyases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacillus subtilis pectin lyase 1A can be used in textile manufacturing, and biotechnology and participates in the eliminative cleavage of pectins.
Publications
Publications (0)
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Publications
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