Base-based modification of nucleosides and nucleotides can be achieved by introducing different chemical groups into the molecular structure of the nucleoside or nucleotide by utilizing organic synthesis. For example, different functional groups are introduced at specific positions by chemical reactions such as esterification, substitution, and oxidation reactions. Another approach is by introducing a specific chemical functional group on a starting molecule and then converting it to the target modifying group using a chemical reaction. For example, by introducing an acetone or allylacetone functional group, it can be converted to an amino group or a hydroxyl group after a selective chemical reaction. By modification, the properties such as solubility, stability, and affinity can be changed, thus improving the efficacy and bioavailability of drugs. In addition, modified nucleosides and nucleotides can be used for labeling molecules, probe design, and biosensors, providing a powerful tool and platform for scientific research.
The key technologies of this service are organic synthesis-based modification methods, specifically halogenation, N-conjugation, and azotation, which utilize chemical reactions such as esterification, substitution, and oxidation to introduce functional groups at specific positions on nucleosides and nucleotides, thereby altering their properties for enhanced stability, solubility, and affinity.
CD BioGlyco is a leading company providing specialized nucleoside & nucleotide Modification services. With innovation and excellence in mind, we are committed to providing high-quality, customized modification solutions to our clients. Our modification services include halogenation, N-conjugation, and azotation.
The process begins with a detailed discussion of your project requirements. Our team of specialists will analyze your specific needs, review the target molecular structure, and develop a tailored modification plan.
Clients provide the necessary nucleic acid samples, ensuring they meet the required purity and concentration standards for the modification reaction.
Our expert chemists execute the base modification using advanced techniques, such as the palladium-catalyzed N-arylation method or other proprietary chemistries, to introduce the desired functional group onto the nucleobase.
The modified products undergo rigorous purification processes to remove unreacted starting materials and by-products. Each batch is then subjected to a comprehensive suite of quality control tests, including mass spectrometry and HPLC, to confirm identity, purity, and integrity.
The purified, high-quality modified nucleosides or nucleotides are delivered to you with a detailed report, including all relevant data. Our scientific support team remains available for any post-delivery questions or assistance.
DOI.: 10.3389/fchem.2022.1051525
Journal: Frontiers in Chemistry
IF: 4.2
Published: 2022
Results: This expansive critique interrogates nucleobase-modified nucleotides—synthetic analogues harboring altered nucleobases devoid of classical hydrogen-bonding functionalities—and their paradigm-shifting implementations across biochemistry, synthetic biology, and therapeutics. The authors methodically assess how such analogues subvert entrenched doctrines of DNA replication fidelity, establishing hydrogen bonding's nonessentiality for polymerase catalysis. Rather, geometric congruence, hydrophobic forces, and π-orbital stacking dictate enzymatic assimilation. The treatise spotlights three pivotal domains: (1) mechanistic revelations concerning polymerase function via analogue deployment as molecular probes; (2) synthetic biology breakthroughs encompassing genetic alphabet expansion through engineered base dyads (exempli gratia Ds:Px) and evolved polymerases accommodating unnatural nucleotides; and (3) therapeutic promise epitomized by nucleoside variants like 5-NIdR, which potentiate temozolomide's anticancer synergism through translesion DNA synthesis inhibition. These innovations crystallize nucleobase modifications' protean utility in reconfiguring genetic engineering, pharmacotherapeutic discovery, and oncological intervention.
Our base modification services enable precise alterations—such as halogenation, methylation, or bioorthogonal tagging—to optimize stability, binding affinity, and therapeutic functionality of nucleic acid building blocks. To expand these capabilities into de novo molecular construction, we offer integrated Nucleoside & Nucleotide Synthesis Services, covering:
CD BioGlyco provides base-based nucleoside & nucleotide modification services based on our leading platform and expertise. Our efficient workflow and strict quality management system ensure the on-time delivery of projects and help our clients achieve success in drug discovery, bioscience research, and other fields. If you need base-based services, please feel free to contact us for more detailed information.
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