Fluoro-bases Modification Is a Promising Modification Method

Fluorine has unusual properties such as small molecules, hydrophobicity, and high electronegativity. A highly electronegative fluorine atom can replace the 2'-OH group of ribose, resulting in a C3'-endo (RNA-type) conformation of the 2'-F-ribonucleoside ring in oligonucleotides. The properties of the fluoro bases modification make it especially suitable for the design of highly effective siRNAs. At the 2'-ribose position, which allows for position-independent incorporation into both strands and locks the sugar in the RNA-compatible C3'-endo conformation, respectively. Fluorine modification increases the RNA affinity of modified siRNA guide strands for their targets and enhances the stability of siRNA duplexes. Neither this increase in affinity nor pairing strength compromised siRNA function and was well tolerated. This modification exhibits greater nuclease stability, significantly reduces immune stimulation in vitro models, and is a promising modification in siRNAs.

Fig.1 The monomer of 2'-fluoro. (Chen, et al., 2019)

Oligonucleotide-based Fluoro Bases Synthesis and Modification Services at CD BioGlyco

• Fluorine Base Modification Design

CD BioGlyco designs precise fluorine-modified base structures based on customer needs and specific applications.

• Synthetic DNA/RNA Oligonucleotide Sequences

CD BioGlyco uses well-established chemical synthesis methods to synthesize DNA, RNA, or oligonucleotide sequences containing fluoro-base modifications.

• Characterization and Analysis

CD BioGlyco provides meticulous characterization and analysis services for synthetic fluorobase-modified oligonucleotide samples, including high-performance liquid chromatography (HPLC) analysis, mass spectrometry, and nuclear magnetic resonance (NMR) analysis.

• Purification and Refinement

We use various purification methods such as gel electrophoresis and affinity chromatography to purify and refine the synthesized fluoro base-modified oligonucleotide samples to remove impurities.

• Quality Control

We provide comprehensive quality control services to ensure the quality of synthesized fluorobase-modified oligonucleotide samples, including sequence verification, purity testing, confirmation of end modifications, etc.

In addition, CD BioGlyco also offers other Oligonucleotide-based Bases Modification Services, including Affinity-plus Base, Inosine, 2'-O-methoxy-ethyl Bases (2'-MOE), and 2'-O-methyl RNA Bases.

Fig.2 Oligonucleotide-based fluoro bases synthesis and modification services. (CD BioGlyco)

Applications

• Imaging Agents: Fluoro base modifications are often utilized in the development of imaging agents for techniques like positron emission tomography (PET) and magnetic resonance imaging (MRI), enabling better visualization and diagnosis of diseases.
• Molecular diagnostics: Fluoro base modification can be used in the design of oligonucleotide probes for molecular diagnostic applications.
• Biosensors: Oligonucleotides modified with fluoro bases can be used to construct highly sensitive and specific biosensors to detect specific molecules or targets.

Advantages

• CD BioGlyco has extensive experience in the field of oligonucleotide synthesis and modification and can provide flexible base synthesis and modification services of different types.
• CD BioGlyco is the world's leading biotechnology company, with advanced laboratory facilities and biotechnology equipment, which can provide accurate and reliable base modification services.
• We provide a full range of real-time after-sales services, allowing you to keep track of your order status anytime and anywhere.

CD BioGlyco is committed to offering mature synthesis, base modification, and purification services for the oligonucleotide. Please feel free to contact us if you want to quickly obtain oligonucleotide base modification services, we are your best choice.

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