In a trans-sialylation reaction facilitated by Tr15, sialic acid from CGMP is moved using the whey-derived LacNAc to produce 3'SLN. This is a useful and cost-effective manufacturing method. In addition, natural resources can be used to produce 3'SLN.

Introduction of 3'SLN

Five monosaccharides serve as the building blocks for the neutral and anionic species that make up human milk oligosaccharides (HMOs). These constituents include L-fructose (Fuc), D-glucose (Glc), D-galactose (Gal), N-acetylglucosamine (GlcNAc), and N-acetylneuraminic acid (or sialic acid; NeuAc). Sialic acid, one of the specific components found in HMOs, is essential for the growth of neurons, the development of the brain, neuronal transmission, cognition, and synaptogenesis.

Fig.1 The structure of 3'SLN. (Duman, et al., 2021)

A class of glycosyltransferases known as sialyltransferases generates sialylated glycans in mammalian systems. Due to the absence of organelle membrane structures, it is notoriously challenging to produce mammalian sialyltransferases in Escherichia coli systems. Therefore, LacNAc was originally generated and purified directly from whey in the majority of current research. The sialic acid from CGMP was then transferred using the whey-derived LacNAc in a trans-sialylation process catalyzed by Tr15 to create 3'SLN. This is a practical and affordable production technique. Additionally, 3'SLN can be obtained from natural sources, but there is no acceptable method for meeting the need on a big scale.

Fig.2 The mechanistic model of synthesis 3'SLN. (Karimi Alavijeh, et al., 2022)

3'SLN Production Services at CD BioGlyco

CD BioGlyco has a professional and advanced production platform, which provides the service of producing HMOs via Chemical Methods, Chemoenzymatic Methods, Biocatalytic Methods, and extraction from natural sources. Based on the platform, we have the ability to produce 3'SLN of different scales and grades. Producing by extraction or synthesis from an artificial/natural mediate are the most two commonly used methods. Moreover, CD BioGlyco provides HMO Profiling Services for clients to study HMO. These customized services provide a great help to our clients' HMO research.

Advantages of Us

• We have a great service team for helping customers with questions we have the ability to solve.
• We have an advanced synthetic platform to produce high-quality 3'SLN according to the client's needs.
• According to the client's needs, we can provide different grades and scales of 3'SLN.
• Our outstanding scientists could provide professional and suitable solutions for customers' inquiries.

CD BioGlyco provides a great production department, if you are interested in our 3'SLN customized production service, please feel free to contact us for more detailed information. We look forward to inquiries from around the world and our research scientists will do their best to help our clients solve their problems and meet their needs in the field of HMO research.

References

1. Duman, H.; et al. Potential applications of endo-β-N-acetylglucosaminidases from bifidobacterium iongum subspecies infantis in designing value-added, next-generation infant formulas. Frontiers in Nutrition. 2021, 8: 646275.
2. Karimi Alavijeh, M.; et al. Bioactives from whey: a sustainable approach to enzymatic production of sialyl-N-acetyllactosamine. ACS Sustainable Chemistry & Engineering. 2022, 10(19): 6265–6275.