3'-Galactosyllactose (3'GL) has excellent biological activities, such as anti-inflammatory, regulating the immune system and the balance of intestinal flora. It has shown potential and broad market prospects in the infant formula industry and the medical industry. Through competent assistance by our researchers and the fastest delivery of high-quality 3'GL products at fair prices, CD BioGlyco has become a "preferred supplier" of many clients.

3'GL with a Special Oligosaccharide Structure

Human milk oligosaccharides (HMOs) are low-polymer sugars that cannot be digested by the gastrointestinal tract but can be hydrolyzed by colonized microorganisms in the intestinal tract. They produce small molecular organic acids, nourish intestinal epithelial cells, maintain the intestinal mucosal barrier, and resist the invasion of pathogenic microorganisms while reducing the pH value of the colon and promoting the absorption of calcium, magnesium, iron, and other minerals. Some functional HMO structures with elevated expression levels in mammalian colostrum form different galactosyl-lactoses based on the extension of lactose, such as 3'GL (beta-D-Galp-(1-3)-beta-D-Galp-(1-4)-D-Glc). The development of science and technology and the exploration of scientific researchers led to the discovery of the structure and some functions of 3'GL, which are expected to be applied to human food and medicine.

Fig.1 Six simple oligosaccharide structures including 3'GL. (Zuurveld, et al., 2020)

The Exploration Process of 3'GL

Although 3'GL has been isolated from bovine colostrum before, it was isolated from human milk glycan for the first time through cycle chromatography by Donald and Feeney in 1988. Later, it is separated from bear, camel, reindeer, elephant, and other mammals' milk. As a special oligosaccharide structure in breast milk, 3'GL has many beneficial and positive effects on infant health, such as regulating innate immunity and cultivating healthy intestinal microflora. Recently, some studies have shown that 3'GL may attenuate NF-kB inflammatory signaling, which has an anti-inflammatory effect on human intestinal epithelial cells in vitro. 3'GL has potential clinical research value as a strong physiological anti-inflammatory agent in human colostrum and early milk.

Key Technologies

CD BioGlyco has accumulated a variety of Production Solutions to meet changing client needs, including but not limited to the following technologies:

• Separation and purification technology

Various high-grade standards and fully optimized separation routes.

• A one-stop solution for enzymatic synthesis

Selection and identification of suitable enzymes, such as galactosidase.

Optimal enzyme expression levels.

• Microbial Fermentation

A complete and mature service product line from strain development to mass manufacturing.

• Combination of multi-disciplinary technologies

Including protein engineering, metabolic engineering, and synthetic biology strategies and tools.

3′GL Production Service: A Comprehensive Guide

CD BioGlyco offers a comprehensive range of 3′GL production services tailored to the unique needs of the industry. We provide both chemical and enzymatic synthesis options, allowing for flexibility in scale and purity.

• Enzymatic Synthesis: This method relies on specialized enzymes, such as glycosyltransferases or glycosidases, to catalyze the transfer of a galactose residue to a lactose acceptor. The enzymatic pathway is particularly well-suited for creating specific glycosidic linkages, ensuring high regioselectivity and yield. Our expertise in enzyme engineering and optimization allows us to fine-tune reaction conditions for maximum efficiency and scalability, producing gram- to kilogram-scale quantities with high purity.
• Chemical Synthesis: Chemical synthesis provides a robust and scalable alternative, particularly for projects that require precise control over reaction parameters. Our team of expert chemists has optimized this process to achieve high yields and purity on a gram scale, utilizing readily available and cost-effective starting materials.

Our services include:

• Custom Synthesis: Tailored production for specific research applications, including isotope labeling or modifications for advanced studies.
• Small-scale research production: Providing high-purity 3′GL for academic research and proof-of-concept studies.
• Large-scale industrial production: Scalable solutions for commercial applications in food, pharmaceutical, and nutraceutical industries.

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With rich production experience of oligosaccharides, CD BioGlyco provides you with high-quality services for 3'GL production. If you are interested in our services, please contact us feel free.

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