Indispensable Adenosine Triphosphate (ATP)

In cells, ATP is an efficient central link - the exchange currency - between the processes that produce energy and those that require it. The synthesis, hydrolysis, or transfer of terminal phosphate groups are involved in these processes. In general, ATP produced during cellular metabolism mainly comes from the catabolism of glucose, which involves three processes: glycolysis, tricarboxylic acid cycle, and oxidative phosphorylation.

Fig.1 ATP synthesis in oxidative phosphorylation. (Wikipedia)

Different Methods to Synthesize ATP

The organism is the production workshop of natural ATP, while most of the ATP used in the research and therapeutic fields is biosynthesized or chemically synthesized in vitro. The most famous chemical methods are the Yoshikawa protocol and the Ludwig-Eckstein method which can achieve moderate product yields. Using adenosine as a substrate, one-step synthesis of ATP by biocatalyst is a mature method for large-scale production. Adenosine is a cheap commercial reagent, and modified adenosine can also be obtained by enzymatic reaction with a high yield. The yield of ATP synthesis by microorganisms is also extremely high. It has been reported that the yield is close to 100% by generating ATP from adenosine by immobilized Saccharomyces cerevisiae.

ATP Production Service at CD BioGlyco

CD BioGlyco has mature ATP synthesis technology and large-scale production capacity. With efficient production processes, we are dedicated to supporting our customers to obtain desired ATP products economically. You can directly apply our ATP products to various studies to ensure the smooth progress of your research.

Fig.2 Different strategies to produce ATP in vitro. (CD BioGlyco)

• ATP in signal transduction: When the kinase phosphorylates the protein, the activated protein regulates various signaling pathways in the cell. ATP is the substrate of many kinases, and the regulation of kinase activity depends on the presence of the magnesium-ATP complex. ATP also acts as a trigger for the release of intracellular messengers. CD BioGlyco provides you with high-purity ATP products to support your research on cell signal transduction, as well as the development of analgesic therapies and anesthetic supplementary therapeutics.
• ATP in DNA/RNA synthesis: ATP is an essential nucleoside triphosphate monomer for RNA synthesis. In DNA synthesis, ATP is converted to deoxyribonucleotide (dATP) by removing one of the oxygen atoms in ribose. CD BioGlyco customizes the direct raw materials for your synthetic genes.
• ATP in purinergic signaling: ATP can induce a variety of purinergic responses such as control of autonomic nervous function, pain, vascular tone, and glial interactions. CD BioGlyco enables you to apply ATP in relevant research and the discovery of therapeutic agents.
• ATP in neurotransmission: The brain consumes almost 13% of the total energy produced by the human body. This large amount of energy is used to maintain ion concentration for neuronal signaling and synaptic transmission. CD BioGlyco brings ATP production service to meet your in-depth study of neurotransmission mechanisms, as well as the development of related therapeutic agents.

Applications

• Research on cell signaling.
• Development of analgesics.
• Development of supplementary therapeutic agents for anesthesia.
• Development of safe pulmonary vasodilators.

Highlights

• Safe chemical synthesis method for ATP production.
• Efficient enzymatic method for ATP production.
• Immobilized microbial technology for ATP production with high yield.
• Medium-scale and large-scale production capacity.
• Rigorous production process and fine purification technology.

CD BioGlyco is a global leader in developing reagents for laboratory research, industry, and diagnostic monitoring. Our Glyco™ Synthesis Platform has helped scientists create many achievements. If you are interested in our ATP production service for clients or Nucleotide-based Production Service, please feel free to contact us immediately. Look forward to becoming your professional assistant.