Arachis Hypogaea Lectin Production Service
Arachis hypogaea Lectin (PNA) Production Service at CD BioGlyco
The biological activity of PNA plays an important role in cell recognition, inflammatory response, blood clot formation, etc., which interacts with sugar molecules on the cell surface and participates in a variety of physiological and pathological processes. PNA has been widely used as a tumor marker because it specifically binds to the β-D-Gal(1-3)-D-GalNAc residue of the Thomson-Friedenreich antigen. CD BioGlyco has a mature Plant Lectin Production process, and we provide professional PNA production services.
- PNA extraction and purification service
- Grinding: PNA is a plant-derived protein, and we select healthy and high-quality peanut seeds for grinding.
- Extraction: We mix peanut powder with an appropriate buffer solution such as phosphate buffer or tris buffer to extract PNA. Stirring or shaking the mixture at a low temperature for a while enhances the extraction effect.
- Centrifugation and filtration: We centrifuge the extract to remove suspended solid particles and impurities, and then further filter the extract using filter paper or microporous filters to remove fine particles.
- Precipitate formation and separation: We add a suitable precipitant (such as ammonium sulfate or polyethylene glycol) to the extraction solution to form a PNA precipitate, and then separate the PNA from the extraction solution by centrifugation.
- Concentration: We redissolve the PNA precipitate in an appropriate buffer solution, and then use concentration techniques to concentrate the PNA in the solution.
- Column chromatography: We use column chromatography technology to further purify the concentrated PNA solution and remove other impurities, such as ion exchange chromatography, gel filtration chromatography, or affinity chromatography.
- PNA activity assay service
PNA mainly exerts its biological activity by interacting with specific sugar molecules on the sugar chain, which binds with high affinity to the β-D-galactose structure on the cell surface or in solution. In addition, PNA has an agglutination effect, which brings red blood cells or other cells together to form agglutinates. We provide a high-level activity assay for purified PNA to determine its ability to bind to specific sugar chain structures. Commonly used methods include hemagglutination test, agglutination test, glucose inhibition test, etc.
- Protein analysis service
We perform protein analysis on purified PNA using analytical techniques (such as SDS-PAGE, western blot, mass spectrometry, etc.) to determine parameters such as purity and molecular weight.
- Product inspection service
We perform inspection and quality control on the final PNA products, using various analytical techniques (such as protein concentration determination, bacterial contamination detection, etc.) to ensure product purity, activity, and consistency.
- Custom service
CD BioGlyco offers many types of PNA Products. Moreover, we provide custom PNA product development services according to client needs, including modifying, labeling, or combining PNA with other proteins/molecular structures to meet specific research or application needs.
- Mass production and supply service
We provide mass production and supply for various types of PNA products. We are committed to providing high-quality, high-purity PNA products to meet client needs.
Fig.1 PNA production service. (CD BioGlyco)
Publication
Technology: Biosensor
Journal: Biosensors and Bioelectronics
IF: 12.545
Published: 2019
Results: The authors constructed a label-free biosensor using PNA for the detection of T antigen, monitoring the formation of complexes between PNA and T antigen in glycoproteins by electrode impedance. The PNA biosensor was used to analyze serum samples from various cancer patients, the results showed that T antigen was highly expressed in skin cancer and breast cancer, and low in kidney cancer, liver cancer, and rectal cancer. The biosensor response was highly selective.
Fig.2 Construction of PNA biosensor and detection of T-carrying glycoproteins by electrode impedance. (Rangel & Silva, 2019)
Applications
- Analysis of cell surface sugar chains: PNA specifically binds to Galβ1-3GalNAc and is used as a probe for cell surface sugar chains, which is used to detect and identify changes in sugar chains on the cell surface, such as the recognition and expression differences of blood group antigens.
- Sugar chain structure research: PNA is used to study and analyze complex sugar chain structures. By combining with specific sugar chain structures, sugar chains are separated, purified, and analyzed, and their functions and biological significance are further studied.
- Cell adhesion and migration research: PNA has agglutination activity and is used to study cell adhesion and migration. By binding to specific sugar molecules, it may affect interactions between cells, adhesion between cells and matrix, and regulation of cell migration.
Advantages
- CD BioGlyco has leading R&D technology, that is committed to providing high-purity, active, and stable PNA products.
- CD BioGlyco provides custom PNA production services according to client needs. We have sufficient resources and production capacity to meet large-scale PNA production.
- CD BioGlyco has an experienced and high-quality technical team, which provides stable and reliable PNA product quality and fast delivery.
CD BioGlyco has top-notch research teams and professional technology to support diverse Lectin Production processes. We strictly follow quality control standards to ensure the stability and consistency of PNA product quality. Please feel free to contact us if you would like to inquire about specific production details.
References
- Rangel, M.G.; Silva, M.L.S. Detection of the cancer-associated T antigen using an Arachis hypogaea agglutinin biosensor. Biosensors and Bioelectronics. 2019, 141: 111401.
- Sun, J.; et al. Purification and identification of a natural lectin from the seed of peanut Arachis hypogaea. The Open Materials Science Journal. 2011, 5(1).
