Properties
Description
Canine lipopolysaccharide-binding protein (LBP) ELISA kit is an enzyme-linked immunosorbent assay for the quantitative detection of canine LBP in samples and its detection range is 50-100 µL.
Assay Principle
Canine lipopolysaccharide-binding protein (LBP) ELISA kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique. Monoclonal antibodies against canine LBP are precoated on the microplate. After the sample and biotinylated antibodies are added to the plate and allowed to react, the plate is washed with PBS or TBS. Then, a peroxidase-conjugated avidin is added and allowed to react, followed by thorough washing with PBS or TBS. Subsequently, a TMB substrate is added for color development. TMB turns blue in the presence of the peroxidase enzyme and is converted to the final yellow color under acidic conditions. The intensity of the color is directly proportional to the concentration of the target factor in the sample.
ELISA Type
Sandwich ELISA kit
Assay Type
Sandwich ELISA kit
Species Reactivity
Canine
Components
Pre-coated 96-well plates; Standard; Concentrated biotinylated antibody; Concentrated ABC (avidin-biotin-peroxidase complex); Enzyme-conjugate diluent ABC; Antibody diluent; Standard diluent; Concentrated wash buffer; Chromogen solution A; Chromogen solution B; Stop solution
Sample Type
Serum, plasma, cell culture supernatant and tissue samples
Detection Method
Colorimetric, OD 450 nm
Precision (Intra-Assay)
CV <8%
Precision (Inter-Assay)
CV <10%
Cross-reactivity
No significant cross-reactivity or interference was observed.
Applications
Canine lipopolysaccharide-binding protein (LBP) ELISA kit can be used to measure the level of canine LBP in serum, plasma, cell culture supernatant and tissue samples.
