Covalent Conjugation Strategy with Surface-Expressing Groups on Cells

The cell surface represents a complex chemical environment composed of proteins, carbohydrates, lipids, and other molecules. A variety of functional groups, such as amine groups and thiol groups, are naturally present on the cell surface, which facilitates direct covalent conjugations with appropriate chemical tools. In addition, artificially introducing groups onto the cell surface allows selective and specific modifications at the desired site. Chemical conjugation enables the introduction of unnatural functionalities, such as fluorophores, into cell surface glycans.

Fig.1 Covalent conjugation strategies with surface-expressing groups on cells. (Wang, et al., 2023)

Chemical Conjugation-based Cell Surface Glycoengineering Service at CD BioGlyco

At CD BioGlyco, we provide chemical conjugation-based cell surface glycoengineering services that cover the currently available chemical engineering tools for cell surface glycoengineering, including bio-orthogonal aldehyde-amine reaction, diels-alder reaction, click chemistry, hydrazone ligation, cyanogen bromide (CNBr) activation, periodate oxidation, Staudinger ligation, maleimide, fluorophore, biotin, chemical crosslinker.

Applications

• A variety of conjugation chemistry methodologies for the activation of polysaccharides have been used to couple carbohydrate antigens to protein carriers.
• The conjugation of various functional groups and biomolecules on the cell surface contributes to better functionality and further development for live cell imaging, drug delivery, control of cell surface interactions, cell tracking, and sensing biological environments in vitro and in vivo.
• Cell membrane glycoengineering has been widely applied in the endowment of cells with new abilities, glycoengineered membrane self-assembly servers as vehicles to coat nanoparticles, in vivo cell tracking, metabolic glycan labeling, cell and drug delivery, and single-cell encapsulation.

Advantages

• Among a variety of cell modification strategies, chemical engineering represents a reliable and straightforward approach to improving their therapeutic potential.
• Compared with genetic engineering or biological engineering approaches, chemical engineering methods have a relatively lower demand for deliberate manufacturing control and are more cost-effective and easier for scale-up production.
• As highly efficient in vitro glycoengineered expression platforms, chemical conjugation-based cell surface glycoengineering technologies have high flexibility and cost-effectiveness.

With proven experience in glycoscience, CD BioGlyco has accumulated substantial experience and expertise in cell line glycoengineering. By assembling top-class scientific experts and integrating state-of-the-art technologies, CD BioGlyco has established efficient Cell Surface Glycoengineering Platforms based on our Cell Surface Glycoengineering Technology that provides several custom services such as Cell Surface Glycoengineering Services. Our expression platforms provide custom functions to our client's needs. If you are interested in our services, please feel free to contact us for more information.

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