CD BioGlyco is the preferred biotechnology company of isotope labeling of glycans for the worldwide research community. We look forward to cooperating with you and becoming your research assistant in the field of glycomics.

Background

The pathogenic mechanism of many diseases lies in changes in the quantity and/or quality of complex carbohydrates. Mass spectrometry (MS) methods are very promising in monitoring and detecting these significant biological changes. The isotopic labeling method is helpful to accurately quantify the level of released glycans. Quantitative derivatization of free reducing glycans through a variety of methods is the basis of differential isotope labeling of glycans. Isotopic labeling strategies include derivatization of glycan main chain by permethylation with reagents containing heavy or light isotopes, or reductive amination of the reducing end of glycans with light or heavy isotope labeling reagents.

Glycan reductive isotope labeling has been reported as a new glycomics method. Free glycans can be derived by reductive amination of stable isotope labels [¹²C₆]-aniline and [¹³C₆]-aniline with differential coding. And two samples were labeled as light and heavy, respectively, which were recovered by reverse phase chromatography and quantified according to UV-absorbance and relative ion abundance. This technology provides necessary advances in the field of glycomics.

Fig.1 Strategy for using glycan reductive isotope labeling to analyze glycan. (Xia, 2009)

Labeling Strategies

CD BioGlyco has developed several quantitative strategies that can reliably and sensitively monitor glycan changes and these strategies utilize a variety of separation and MS techniques. Services we provide include but are not limited to:

• Quantitative glycomics through stable isotopic labeling. We combine different isotopic species to chemically similar analytes to produce identifiable quality differences, allowing simultaneous MS analysis of multiple samples.
• Stable isotopic labeling via permethylation. This strategy is often used in conjunction with LC-MS to quantify glycans derived from glycoproteins and human serum.
• Stable isotopic labeling by reductive amination. Reductive amination reagents such as PA, 2-AA, and 2-AB have been used in combination with stable isotope-labeled reducing reagents, which can be used for relative quantification and comparative quantification of glycans.

Applications

• Application in quantitative glycomics
• Application in high-throughput screening of comparative glycomics
• Application in proteomics, lipidomics, and metabolomics

Advantages of Us

• Simple labeling technologies
• Simplified MS approaches
• Assistance with technical inquiries
• Fast, reliable, and inexpensive

CD BioGlyco has a professional research team covering the fields of synthetic, organic and analytical chemistry, NMR and MS methodology. With our vast knowledge and experience, we can help researchers meet their unique requirements. Please contact us directly if you need technical support.

Reference:

1. Xia, B.; et al. Glycan reductive isotope labeling for quantitative glycomics. Analytical Biochemistry. 2009, 387(2):162-170.