Dolichos Bifloru Lectin Production Service
Dolichos bifloru Lectin (DBA) Production Service at CD BioGlyco
DBA is extracted and purified from the seeds of the leguminous plant D. biflorus. DBA is a heterotetrameric glycoprotein composed of four subunits of equal size. It has binding activity and is used as a marker for primordial germ cells and spermatogonia to indicate their differentiation status. It also determines the secretion status of individuals with type A blood through hemagglutination inhibition and is used to detect blood types. CD BioGlyco has many years of experience in Plant Lectin Production and purification, and we provide efficient DBA production service.
- Plant extracts
D. biflorus seeds contain DBA, a phytohemagglutinin that is a natural protein that binds to specific sugar molecules, which has carbohydrate specificity for β-N-acetylgalactosamine.
- We grind mature D. biflorus seeds to increase cell wall disruption and release of soluble proteins.
- We use an appropriate buffer to extract the seed powder, dissolve DBA into the extraction solution, and separate the particles through centrifugation and other operations.
- We purify the extracted mixture through column chromatography or other separation techniques, including ion exchange chromatography, affinity chromatography, etc. By choosing an appropriate purification method, we separate the target lectin from other proteins and impurities.
- We wash the purified lectins to remove residual impurities and buffers. Appropriate concentration techniques are then used to increase the concentration of the lectin to the desired level.
- We perform protein identification (such as SDS-PAGE gel electrophoresis) on the produced DBA and conduct activity tests to determine its agglutination ability. Activity testing typically involves experiments with binding to specific glycogen-specific antigens.
- We will package the purified and characterized DBA appropriately, such as lyophilization or cryopreservation, which ensures the stability and activity of the product during storage.
- Conjugate preparation
In addition, CD BioGlyco also provides conjugate preparation for the produced lectins, and we have developed a diverse range of DBA-conjugate Products.
Fig.1 DBA production service. (CD BioGlyco)
Publication
Technology: Immunohistochemical staining
Journal: Andrology
IF: 4.456
Published: 2016
Results: The authors screened several markers to differentiate and study spermatogonia populations in situ and in vitro using immunohistochemical staining. The results showed that undifferentiated spermatogonia were not labeled by DBA (Fig. 2A), DBA staining intensity (brown precipitate), and labeled cell area increased in pubertal tissue (Fig. 2B), reaching a maximum in active seminiferous tubules (Fig. 2C). This demonstrated that DBA lectin was used to label and isolate undifferentiated spermatogonia from a variety of animal species.
Fig.2 DBA lectin-stained testicular tissue sections. (Escada-Rebelo, et al., 2016)
Applications
- Sugar chain identification: DBA binds to the sugar chain of N-acetylgalactosamine structure on the cell surface, which determines whether this type of sugar chain exists on the cell surface and further studies its functions in cell recognition and interaction.
- Cancer research: DBA is used to detect specific sugar chain markers on the surface of tumor cells, which helps to understand the molecular composition and phenotype of tumor cells and provides a basis for early research of tumors.
- Diabetes research: DBA is used to evaluate and explore specific sugar chain modifications of islet cells and insulin receptors and their functions in glucose metabolism and insulin signaling.
Advantages
- Reliable supply chain: CD BioGlyco has established a stable raw material supply chain to ensure a sufficient supply of materials required for DBA production.
- High purity: We use efficient purification methods and technologies to ensure the high purity and low impurity content of DBA products.
- Flexible service model: We flexibly adapt to client needs and provide various DBA production from small-scale research to large-scale industrial usage.
CD BioGlyco has an effective process flow that achieves high-volume DBA production, we respond quickly to client orders and deliver DBA products on time. Moreover, we also provide many types of Lectin Production Services. Please feel free to contact us if you need high-quality DBA production services.
Reference
- Escada-Rebelo, S.; et al. Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin. Andrology. 2016, 4(6): 1159-1168.
