Erythrina cristagalli Lectin (ECA) Production Service at CD BioGlyco

ECA is derived from the E. cristagalli plant, which belongs to the legume family and contains ECA in its seeds and other tissues. ECA binds to carbohydrate structures in mammalian cell membranes and serum glycoproteins, specifically recognizing galactose/N-acetylgalactosamine (Gal/GalNAc). ECA is used in the glycobiology and isolation of human NK cells and has a mitogenic effect on human peripheral blood T lymphocytes.

• High-quality ECA production service

CD BioGlyco has established one-stop Plant Lectin Production solutions. We provide large-scale ECA production service, which is often used in biological research, glycology research, and biochemical experiments. The production of ECA mainly involves the following steps.

• Tissue sample extraction
  We select appropriate tissue samples such as seeds, roots, leaves, or stems from harvested E. cristagalli plants and perform lectin extraction. For ECA, seeds are the most commonly used source. We extract ECA from tissue samples using appropriate extraction methods such as grinding, soaking, and centrifugation.
• Lectin purification
  We purify the extract through multiple precipitations and washing steps using a salt solution such as ammonium chloride, which helps remove non-specifically bound proteins and impurities. Next, we further purify ECA using column chromatography, electrophoresis, or other separation techniques.
• Wash and concentrate
  We wash the purified ECA to remove residual impurities and buffers, and concentrate ECA through mature concentration technology (such as ultrafiltration or centrifugation).
• Activity assay
  We perform protein characterization of the produced ECA to determine their molecular weight and purity. At the same time, we provide agglutination activity determination for purified ECA through a hemagglutination test. ECA is not blood group specific but has an affinity for D-galactose and D-galactopyranoside.

• Diversified ECA product supply service

We have developed Lectin from E. cristagalli and provide reliable product supply service to our clients. In addition, we provide conjugation production service for affinity-purified ECA using special conjugation procedures. For example, we label ECA with fluorescein isothiocyanate (FITC) to provide maximum fluorescence and optimal staining properties for this specific lectin.

Fig.1 ECA production service. (CD BioGlyco)

Publication

Applications

• Separation and purification of sugar chain structures: ECA is used as an affinity chromatography material to help separate and enrich molecules containing specific sugar chain structures.
• Cell identification and labeling: ECA is used as a cell surface marker to help distinguish different types of cells and perform cell isolation. At the same time, ECA is also combined with markers such as fluorescent dyes or gold particles to observe and track specific glycosylation sites under a microscope.
• Sugar metabolism research: ECA is used to study changes in sugar metabolism in cells and organisms, and to explore sugar metabolism pathways related to disease development.

Advantages

• CD BioGlyco has extensive experience in Lectin Production. Our professional R&D team uses advanced bioengineering technology and separation and purification technology to ensure efficient and high-purity ECA production.
• We adopt scientific management methods to continuously optimize processes and improve technology to improve the quality and production efficiency of ECA.
• We provide custom ECA production services based on client needs, including choices of different specifications, purity, and labeling methods.

CD BioGlyco strictly follows production quality control standards, we provide ECA production needs of different scales and ensure product quality and consistency. We respond quickly to client needs, please feel free to contact us if you need reliable ECA products.