Fucosyltransferases (FUTs)-based Cell Surface Chemoenzymatic Glycoengineering Service at CD BioGlyco

Many glycans associated with cell surface receptors contain N-acetyl lactosamine (LacNAc) at the penultimate or terminal position, which regulates receptor dimerization and signaling, and is involved in mediating key cellular processes such as apoptosis, proliferation, and differentiation. CD BioGlyco uses FUTs to transfer fucose to LacNAc on the cell surface and provides professional FUTs-based cell surface chemoenzymatic glycoengineering services.

• Chemoenzyme-catalyzed cell modification services

Glycosyltransferases are applied to transfer monosaccharide analogs from nucleotide sugar donors to specific glycan acceptors on the cell surface. CD BioGlyco employs a rapid and highly specific chemoenzymatic approach to perform cell surface glycoengineering services. The α1-3 fucosyltransferase (α1-3 FucT) from Helicobacter pylori has broad substrate tolerance to GDP-fucose analogs modified at the C-5 position. We use α1-3 FucT catalytic transfer of azide- or alkyne-containing fucose to LacNAc-containing cell surface glycans.

• Cell labeling services

FUTs transfer monosaccharides bearing reactive groups such as azides or alkynes from a nucleotide sugar donor to a target glycan acceptor on the cell surface. CD BioGlyco uses bioorthogonal click reactions to perform click links with fluorophores or biotin probes with complementary reactive groups, thereby providing rapid and sensitive cell labeling services.

• Glycosyl modification analysis services

In addition, CD BioGlyco also provides glycosyl modification analysis for modified cell samples. We use a variety of technical methods, such as mass spectrometry, chromatography, and immunoassay, to conduct qualitative and quantitative glycosyl structure analysis.

Fig.1 FUTs-based cell surface chemoenzymatic glycoengineering services. (CD BioGlyco)

Publication

Technology: Bioorthogonal chemistry technology

Journal: Angewandte Chemie

IF: 16.823

Published: 2011

Results: The authors reported a specific two-step chemoenzymatic approach for site-specific chemical labeling of LacNAc-bearing glycans in living cells. The authors treated cells with non-natural monosaccharide precursors, performed fucosylation reaction under the action of α1-3 FucT, labeled the cells with azido or alkynyl groups, and then performed bioorthogonal reactions to achieve specific labeling of glycans.

Fig.2 Schematic diagram of cell surface glycan labeling process. (Zheng, et al., 2011)

Applications

• Cell interactions research: The introduction of specific glycosyl structures on the cell surface through FUTs regulates the interactions between cells, including cell adhesion, cell recognition, and signal transmission.
• Tumor immunity research: Using FUTs to carry out glycosyl engineering on the cell surface changes the glycosyl structure on the surface of tumor cells, thereby regulating the tumor immune response.
• Vaccine research: Using FUTs to carry out chemoenzymatic glycoengineering on the cell surface, specific sugar structures are introduced into the vaccine to enhance the immunogenicity and immune-stimulating effect of the vaccine. which is of great significance for antigen recognition, immune protection effect, and customized design for specific pathogens in vaccine development.

Advantages

• Advanced equipment and technology platform: CD BioGlyco is equipped with advanced experimental equipment and a Cell Surface Glycoengineering Technology Platform. We support efficient and accurate experimental operations and data analysis, ensuring service quality and reliability.
• Diversified substrate modification capabilities: CD BioGlyco performs glycosylation modification on different types of cells, including cell lines, primary cells, and stem cells, to achieve specific modifications for different substrates.
• Rapid response and delivery: We respond quickly to client needs, and provide efficient project management and timely project delivery.

CD BioGlyco provides high-quality, customized FUTs-based cell surface chemoenzymatic glycoengineering services and meets client needs in glycosyl modification and glycobiology research. Please feel free to contact us if you need chemoenzymatic glycoengineering services, we are your best partner.