Properties
Purity
lyophilized powder, stabilized with ∼ 25% (w/w) bovine serum albumin, ≥200 unit/mg protein (enzyme + BSA)
Unit Definition
One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using Heparin, Sodium as substrate for heparinase I.One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using bovine kidney Heparan, Sulfate as substrate for heparinase III.One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 units. Package sizes are sold in units.
Applications
Heparinase I and III Blend from Flavobacterium heparinumhas been used to: • digest heparan sulfate from sheep vitreous • human embryonic kidney cells • glycosaminoglycans in arterial tissue
