The Basic Features of Homarus americanus Lectin

The haemocytes are the source of serum lectin activity. The agglutination activity found in hemolymph serum has at least two components, lobster agglutinin I and II (LAg I and LAg II). LAg I binds mature T-thymocytes. Further explanation, LAg I is a sialic acid binding lectin that circulates as a multimer of 500-700 kDa, composed of 70 kDa subunits. LAg II has a specificity for N-acetyl-D-galactosamine residues (GalNAc).

Homarus americanus Lectin Production Service at CD BioGlyco

CD BioGlyco has advanced technology and a team of professional scientists with extensive experience in Lectin Production. We provide not only Homarus americanus lectin production services including extraction or recombinant synthesis, but also lectin isolation and characterization services.

• Extraction service

Choose the healthy and energetic Homarus americanus, then collect haemolymph collection and preparation plasma. The resultant supernatant (plasma) is stored at -80°C. We use affinity chromatography to isolate and purify lectins. Elute the resulting fractions and remove proteins, before lyophilization and storage.

Our company provides some services to analyze the lectins for insoluble carbohydrates, including sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) or cellulose column.

• Synthesis service

Equipped with a professional scientific team, CD BioGlyco provides high-quality Homarus americanus lectin synthesis service. An expressed sequence tag with a canonical carbohydrate recognition domain (CRD) domain is identified from the lobster transcriptome database. Based on this, the desired gene fragments are cloned using reverse transcription-polymerase chain reaction (RT-PCR) and by rapid amplification of cDNA ends (RACE). Meanwhile, we provide the linking protein structure prediction service.

• Other services

• Amino acid sequencing: Protein bands of interest are excised from gels and submitted for trypsin digestion and amino acid sequencing by mass spectroscopy.
• Haemagglutination (HA) and haemagglutination inhibition (HI): The concentrated lectin fractions are diluted after dialysis with the buffer. The diluted lectin is mixed with washing solution equine, bovine, ovine, caprine, etc. erythrocytes in round-bottom microtitre plates and incubated at room temperature.
• Trypsin digests: Select samples are dialyzed against digestion buffer, concentrated by centrifugation, and processed for trypsin digestion. At the end of the reaction, detection is performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
• Electron microscopy: When sufficient sample is available, purified lectin is examined by negative staining with phosphotungstic acid on a transmission electron microscope.

Fig.1 Schematic diagram of Homarus americanus lectin extraction and analysis. (CD BioGlyco)

Applications of Homarus americanus Lectin

• Homarus americanus lectin has a crucial role in immune recognition and immune effects. Maintains native structure during the purification process.
• Homarus americanus lectin appears as both a ~40 kDa monomer and a ~70 kDa homodimer on non-reduced gels, is readily captured by the mannan-agarose column, and showed preferential elution by N-acetylated monosaccharides.
• Changes in plasma Homarus americanus lectin correlate with the degree of inflammation. Homarus americanus lectin determines what is expected under different physiological and pathological (infectious and inflammatory diseases) conditions.

Advantages of Us

• Our company pays extra attention to the quality of products and services. Each step of the process of extraction or analysis is strictly controlled.
• All experimental animals are safely kept in a compliant environment and fed regularly. We promise that the animals involved in this service are conducted under the rights of animal experimentation, clinical research, and biodiversity and that we comply with relevant international and institutional guidelines.

CD BioGlyco has a production platform to provide clients with a high-performance Natural Lectin Production Service. According to the client's scientific research needs or other needs, we are committed to proposing the perfect solution. Please feel free to contact us if you are interested in Animal Lectins.