Properties
Molecular Weight
210-220 kDa (Subunits: 55-60 kDa)
Purity
ammonium sulfate suspension, ≥2.0 units/mg protein (biuret)
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-L-fucoside to p-nitrophenol and L-fucose per min at pH 5.5 at 25°C.
Form
ammonium sulfate suspension
Applications
α-L-fucosidase from bovine kidney has been used:• to study their ability to alter primary cell transduction• to examine the role of fucose residues of cell surface glycans in adhesion• to investigate its role during fertilization
