α-linked Sialic Acid-binding Lectin Production Services at CD BioGlyco

CD BioGlyco has accumulated extensive experience in the field of Lectin Production. We provide various types of lectin production services, such as Natural Lectins and Recombinant Lectins. In recombinant lectin production, like Sialic Acid-binding Lectin, we provide the whole process of lectin preparation, purification, and analysis. Among them, the production of α-linked sialic acid-binding lectin includes but is not limited to the following.

• Preparation

We mainly prepare α-linked sialic acid-binding lectin based on the process of identifying the source host, constructing the expression vector, and preparing the lectin. We usually use a specific bacterium as the host to construct the protein expression vector and then prepare the target lectin.

• Purification

We use lectin Affinity Chromatography to purify α-linked sialic acid-binding lectin. First, we collect the eluate. Then the eluate is dialyzed several times. Finally, lyophilization is performed to obtain high-purity target lectin.

• Characterization
  • Molecular weight
    Matrix-associated laser dissociation/ionization-time of flight mass spectrometry is currently available for the accurate determination of molecular mass in lectin analysis with high sensitivity and resolution. When using it in tandem with other mass spectrometry techniques, it is also possible to determine the sequence structure of the amino acids in the lectin.
  • Amino acid composition
    Determination of amino acid composition in lectins using an amino acid analyzer-ultraviolet spectrophotometer is an effective method.
  • Coagulant activity
    Coagulant activity is an important property of lectins. There are hemagglutination tests and tube methods for analyzing the coagulant activity of lectins. The core operation of the different experiments is: a sample of lectin is added to red blood cells and the coagulation effect is observed in the mixture at intervals.

• In addition, we offer a semi-antigen inhibition assay and stability assay to test the glycoconjugation and stability of the lectin.

Fig.1 Production services content of α-linked sialic acid-binding lectin. (CD BioGlyco)

Applications

• The α-linked sialic acid-binding lectin is used as an analysis and purification tool for glycoproteins, and is also used to detect selective control of glycosylation during production.
• The α-linked sialic acid-binding lectin is used to separate glycosylated virus particles from glycoproteins.

Advantages

• Our lectin production services are comprehensive, efficient, and stable.
• We provide custom production solutions for our clients according to their needs.
• We have professional and timely pre-sales and after-sales services to ensure client satisfaction throughout the service.

CD BioGlyco provides high-quality, custom, and large-scale lectin production services based on advanced methods and technology. We not only provide unmodified natural lectins but also produce complex recombinant lectins. Please feel free to contact us if you have any further questions.