N-Acetyl-glucosaminyl Transferase-based Cell Surface Chemoenzymatic Glycoengineering Service at CD BioGlyco

Cell surface chemoenzymatic glycoengineering technology is a new method to label glycans by incorporating non-natural sugars into living cells through chemical enzymes, It has the characteristics of high specificity and enhances the visualization of the glycome, which helps researchers to study glycoprotein function in living systems. As a leading global biotechnology company, CD BioGlyco provides excellent integrated N-acetyl-glucosaminyl transferase-based cell surface chemoenzymatic glycoengineering services.

• Substrate customization and modification services

CD BioGlyco designs and synthesizes specific substrates for N-acetyl-glucosaminyl transferase-mediated glycosyl modification, which includes selecting a suitable substrate structure, and changing the sugar chain length, branching degree, and sugar composition of the substrate.

• Enzyme reaction conditions optimization services

CD BioGlyco provides optimization and regulation of enzyme reaction conditions for different substrates and reaction requirements. For example, parameters such as reaction temperature, pH value, substrate concentration, and enzyme addition amount are optimized to achieve efficient glycosyl transfer reactions.

• Cell surface glycoengineering services introduced by enzyme catalysis

NmLgtA is a β1-3-N-acetyl-glucosaminyl transferase from Neisseria meningitidis. In the presence of UDP-6N₃GlcNAc, NmLgtA catalyzes the introduction of GlcNAc from UDP-GlcNAc to the receptor at CD BioGlyco, thereby azide-modified GlcNAc sugars are incorporated into the cell surface glycans.

• Cell imaging services

CD BioGlyco further performs a bioorthogonal reaction on the sugars introduced on the cell surface, we perform a click chemical reaction on the enzymatically treated cells with biotinyne, and then the status of the cells is observed on a microscope using streptavidin-FTCI.

• Cell surface glycan analysis services

CD BioGlyco also provides various analytical techniques (such as mass spectrometry, chromatography, etc.) to conduct structural analysis of modified cell surface sugar groups. Through these analysis methods, information such as the position, type, and relative abundance of glycosyl modifications are determined.

Fig.1 N-acetyl-glucosaminyl transferase-based cell surface chemoenzymatic glycoengineering services. (CD BioGlyco)

Publication

Technology: Bioorthogonal reactions

Journal: Biology Open

IF: 2.643

Published: 2017

Results: The author used β1-3-N-acetyl-glucosaminyl transferase to catalyze the introduction of GlcNAc with an azide group from UDP-GlcNAc to the receptor molecule on the cell surface, and then used the principle of bioorthogonal reaction to combine it with the alkyne group-bearing GlcNAc. The groups performed click reactions to achieve labeling and imaging of living cells.

Fig.2 Labeling and imaging results of live cells. (Almaraz & Li, 2017)

Applications

• Substrate-specific modification: By regulating the interaction between N-acetyl-glucosaminyl transferase and the substrate, the structural characteristics of the substrate, and reaction conditions, directional modification of different substrates is achieved.
• Disease mechanisms research: Use N-acetyl-glucosaminyl transferase to modify cell surface glycosyl groups to simulate or change the sugar structure related to specific diseases, which helps to study the mechanisms of disease and cell signaling.
• Drug delivery system: Through cell surface glycoengineering technology mediated by N-acetyl-glucosaminyl transferase, drugs are combined with specific cell surface molecules and utilize the intracellular metabolic mechanism to achieve targeted delivery.

Advantages

• CD BioGlyco is familiar with the characteristics, substrate selection, and reaction conditions of N-acetyl-glucosaminyl transferase. Extensive expertise and experience are the basis for successful cell surface chemoenzymatic glycoengineering.
• CD BioGlyco has top experimental facilities and technical means to support the development of N-acetyl-glucosaminyl transferase-based cell surface chemoenzymatic glycoengineering, which ensures the smooth conduct of experiments and the delivery of high-quality projects.
• CD BioGlyco has established a complete quality control system to ensure the accuracy and reliability of cell surface chemoenzymatic glycoengineering through strict experimental operation specifications and data verification.

CD BioGlyco actively explores and applies the latest scientific research results and continuously innovates and improves cell surface chemoenzymatic glycoengineering technology. We provide high-quality N-acetyl-glucosaminyl transferase-based cell surface chemoenzymatic glycoengineering services. Please feel free to contact us if you would like to inquire about specific technical service details.