Oligonucleotide-based Linker Modification Service

Applications of Modified Oligonucleotides

Oligonucleotides attached to different chemical groups have become important tools in medicinal chemistry, molecular biology, disease diagnosis, and other fields. After the introduction of modification groups, the properties and functions of the oligonucleotides will be changed and their application scope will be expanded such as changes in the penetration of DNA fragments to cell membranes, changes in resistance to nucleases, and changes in thermal stability. If the chemically active groups are combined into oligonucleotides, conjugants combined with peptides, dyes, and other molecules can be obtained. As a therapeutic approach, oligonucleotide modification can improve the pharmacokinetic and pharmacodynamic properties, and increase the efficiency of oligonucleotide delivery and penetration. Oligonucleotides have powerful functions as primers or probes in the laboratory, and synthesizing modified oligonucleotides with special chemical groups is a key step for the success of the experiment.

Fig.1 Cholesterol-modified oligonucleotide and its application in DNA-encoded chemical library synthesis. (Yang, et al., 2021)

Oligonucleotide-based Linker Modification Service at CD BioGlyco

CD BioGlyco develops different synthesis and modification technologies and provides end-to-end services to help clients select suitable modification solutions to support their research in various biotechnologies.

Amino-based Oligonucleotide Modification Service

Amino linker adds an amino group to the nucleotide, which can cross-link with the NHS ester group. This reaction can be used to attach NHS ester activated ligand molecules, like dyes and biotin, to oligonucleotides.

Biotin-based Oligonucleotide Modification Service

Biotin binds irreversibly to streptavidin and can withstand washing at extreme pH and ionic strength. Biotin-modified oligonucleotides can be widely used in various isolation and detection experiments.

Thiol-based Oligonucleotide Modification Service

Thiol groups is often added to oligonucleotides in the form of disulfide, which avoids damage to the thiol groups by chemical synthesis. By introducing thiol groups, researchers can attach ligands activated by iodoacetamide or maleimide on oligonucleotides.

Alkyne-based Oligonucleotide Modification Service

Acetylene modification involves the attachment of an activated acetylene group to the 5' end of the oligonucleotide which allowing various azide-modified labels on oligonucleotides.

Azide-based Oligonucleotide Modification Service

Azide modification uses NHS ester functional groups to attach azide fragments at the 5' end, 3' end or interior location of the oligonucleotide, and then then further modification can be easily and selectively introduced by bio-orthogonal reactions.

Adenylation-based Oligonucleotide Modification Service

When there is no ATP, adenylazed oligonucleotide can be used as a direct substrate for T4 RNA ligase to covalently link with another single-stranded oligonucleotide, which can be used for 5' end labeling, ribosome assembly, miRNA library construction, etc.

Digoxigenin-based Oligonucleotide Modification Service

Digoxin is a steroid, and organisms other than digitalis usually do not contain antibodies against digoxin, so digoxin labels can avoid the background brought by other labels, and are widely used in various hybrid reactions.

Applications

Synthesis of modified oligonucleotides with specified groups.
Preparation of primers or probes with special functions.
Improvement of cell membrane penetration of DNA.
Improvement of the resistance of nucleic acid to nuclease.
Improvement of the resistance of nucleic acid to nuclease.
Improvement of pharmacokinetic and pharmacodynamic properties of oligonucleotides.

Highlights

Efficient modification process.
Standard synthesis technology and top platform.
Professional experts.
Intimate after-sales service.

CD BioGlyco has expertise in Oligonucleotide Synthesis and Oligonucleotide Modification. we have a strict quality assurance system to ensure product quality and timely delivery. If you are interested in our Custom Sugar-nucleotides Synthesis service, please feel free to contact us immediately for more information.

Reference

Yang, G.Y.; et al. Cholesterol-modified oligonucleotides as internal reaction controls during DNA-encoded chemical library synthesis. Bioconjugate Chemistry. 2021, 32 (4): 667-671.

This service is for Research Use Only, not intended for any clinical use.

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CD BioGlyco is a world-class biotechnology company with offices in many countries. Our products and services provide a viable option to what is otherwise available.

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