As a human milk oligosaccharide, the biological activities of para-lacto-N-neohexaose (pLNnH) and its derivatives have attracted more and more attention from biological science researchers. As a trusted operator, CD BioGlyco provides you with advanced services for pLNnH production.
Human milk oligosaccharides (HMOs) are recognized as having many excellent biological functions that benefit breastfed infants. It has become a popular trend for researchers to focus on synthesizing HMOs and imitating their natural diversity for formulation studies. Oligosaccharides of human milk can be classified into seven groups by the structures of their core oligosaccharides, which include lactose, lacto-N-octaose, lacto-N-neooctaose, lacto-N-hexaose, lacto-N-neohexaose, and so on. pLNnH is a derivative of lacto-N-neohexose, extracted from human milk. Researchers have studied and explored the biological activities and synthesis of pLNnH and its related derivatives to discover the application value of this compound in medicine, food, and other fields.
Fig.1 The structure of pLNnH. (CD BioGlyco)
The research on fucosylated derivatives of pLNnH has also become extensive. Purification of milk samples of a beluga and Minke whales by preparative thin layer chromatography, gel filtration, high-performance liquid chromatography, and ion-exchange chromatography recently. Two new types of oligosaccharides were found in the milk of minke whales, namely sialyl lacto-N-neotetraose and sialyl pLNnH. From a biological point of view, the researchers speculate that oligosaccharides extracted from minke whale milk may act as some anti-infection factors to protect lactating young whales from bacteria and viruses. And a derived structure was extracted from hooded seal milk, Crystophora cristata, which is Fuc(α1-2)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc (monofucosyl pLNnH). There have been reports on the synthesis of fucosylated pLNnH using enzyme mutants, which provides a practical method for the study of future biological applications.
CD BioGlyco has accumulated a variety of Production Solutions to meet changing client needs, including but not limited to the following technologies. It is hereby declared that the pLNnH we offer should only be used as laboratory reagents for research and development.
Various high-grade standards and fully optimized separation routes.
Including fermentation, enzyme screening, and enzyme evolution for all biocatalysis challenges.
A complete and mature service product line from strain development to mass manufacturing.
At CD BioGlyco, we serve as your tactical collaborator in advancing pLNnH-related product development and commercialization initiatives. Our comprehensive service portfolio encompasses custom synthesis, large-scale manufacturing, and analytical solutions, all tailored to bolster your R&D endeavors. From providing gram-scale quantities for preliminary studies to supplying multi-kilogram batches for commercial production, our scalable operational frameworks are specifically engineered to align with your project requirements.
We understand your specific needs, such as desired yield, purity, and application, and design a customized production strategy, whether through enzyme synthesis or microbial fermentation.
For microbial fermentation projects, we will optimize the host strain to maximize pLNnH production. This includes genetic engineering and media optimization. For enzyme synthesis projects, we will select and optimize the specific enzyme and reaction conditions to ensure maximum efficiency and product purity.
Once the production strategy is determined, we will scale up the synthesis process in advanced bioreactors. Our facilities are equipped with advanced systems for precise control of parameters such as pH, temperature, and nutrient levels, ensuring consistent and reproducible synthesis.
After synthesis, pLNnH undergoes rigorous downstream purification processes, including advanced chromatography and filtration techniques to remove impurities and isolate the target molecule. Techniques such as high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and mass spectrometry (MS) are then used to confirm its composition, purity, and quality.
Each batch of pLNnH undergoes rigorous testing to ensure it meets both internal and customer-specific quality standards. The final product is delivered promptly and safely along with a detailed certificate of analysis (CoA).
Scalable and Accessible Production
Our optimized microbial fermentation and enzymatic synthesis platforms allow for scalable production, making high-quality pLNnH accessible from research-grade to industrial-scale quantities.
Decades of Glycoscience Leadership
With many years of experience, our team of seasoned scientists is at the forefront of glycoscience, continuously innovating and refining our synthesis methods.
Dedicated Customization and Tailored Solutions
We pride ourselves on providing customized solutions. Every project is unique, and we tailor our approach to meet your specific technical and commercial requirements, ensuring a successful outcome.
Functional Foods for Digestive Health
pLNnH can be used as a bioactive ingredient in functional food products aimed at improving digestive health and overall well-being in all age groups.
Dietary Supplements for Gut and Immune Support
The immune-modulating and prebiotic properties of pLNnH make it a valuable component in dietary supplements designed to support gut health and immunity.
Fundamental Research and Novel Therapeutics
High-purity pLNnH can be used as a standard for studies on glycobiology, the gut-brain axis, and the development of novel therapeutic strategies.
"The quality of the ultra-pure pLNnH provided by CD BioGlyco was instrumental in validating our recent anti-adhesion assay against multiple enteric pathogens. The Certificate of Analysis was detailed and confirmed the high structural integrity, which significantly reduced our QC burden."
- J.S., Lead Scientist
"CD BioGlyco's ability to transition from a small proof-of-concept order to a large batch, all while maintaining over 97% purity, was highly impressive. The reliability of their supply chain and their transparent communication have been invaluable in keeping our R&D timelines on track."
- A.K., Director of R&D
"The custom linker synthesis on the pLNnH was executed flawlessly, allowing us to develop a novel diagnostic probe successfully. The NMR data they provided gave us confidence in the product from day one."
- P.C., Senior Researcher
CD BioGlyco provides high-quality production services of pLNnH, and we are delighted to work with our clients to help them meet their changing needs. If you want to know more details, please feel free to contact us.
