High-quality Lectin Production Services at CD BioGlyco

CD BioGlyco has been working for a long time on the production of various types of lectins, such as Natural Lectins or Recombinant Lectins. Among the natural lectins, Plant lectins account for a large proportion. At CD BioGlyco, we offer our clients a professional and comprehensive plant lectin production service. Also, we have prepared many Related Products. In the production of Phytolacca americana lectins (PWM), we offer the following services.

• Extraction and isolation services
• Extraction
  PWM is present in the roots of Phytolacca americana. We cut the roots and use a buffer or salt solution to dissolve the powder and extract PWM. Then, after high-speed centrifugation, sedimentation, and filtration, the supernatant is collected and lyophilized to obtain PWM crude.
• Purification
  Using a buffer to dissolve the lectin crude, it is graded for precipitation and salting out, purified, and collected using Gel Filtration Chromatography and Affinity Chromatography.

• Performance testing services
• Molecular weight
  Using gel permeation chromatography to determine the molecular weight of the lectin and sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the molecular weight of the subunit structure of the lectin.
• Structure
  Using circular dichroism, determine the structure of PWM.
• Hemagglutination activity
  Using the hemagglutination method to detect its agglutination activity. Different amounts of PWM solution are added to the wells of the hemagglutination plate, to which erythrocyte suspension is added separately. The coagulant activity of the lectin is observed under the naked eye or microscope.
• Stability
  The activity of PWM is tested at different temperatures to get the results of its thermal stability. Using buffers of different pH to dissolve it and testing its activity separately to get the results of acid-base stability.
• Sugar content
  Using specific monosaccharides as standard sugars, it is determined by anthrone colorimetry.
• Sugar binding specificity
  Using buffer to dissolve PWM, add solutions of different sugars to it and test for hemagglutination activity using hemagglutination assay.

Fig.1 Production content of PWM. (CD BioGlyco)

Publication

Applications

• PWM binds certain neuronal surface glycoproteins and glycolipids, and it is used for the development of neuroanatomical tracers.
• PWM is used to measure cell-cell proliferation responses.
• PWM binds specifically to N-acetylglucosamine and it is used to detect N-acetylglucosamine.

Advantages

• We provide a one-stop service from lectin extraction to analysis, which greatly improves the research efficiency of our clients.
• Our R&D team has professional knowledge in lectin production and custom R&D solutions for our clients.

CD BioGlyco keeps up with the trend of scientific and technological development and provides advanced lectin research and development services to our clients. We Produce Lectins with good stability and high purity to ensure that they effectively support our clients' research activities. We also hope to be the right hand of our clients. Please feel free to contact us for more details without any hesitation if you are interested in our services.

References:

1. Hayashida, M.; et al. Crystallization and preliminary X-ray analysis of lectin C from the roots of pokeweed (Phytolacca americana). Acta Crystallographica Section D. 2003, 59: 1249-1252.
2. Fujii, T.; et al. Structures of two lectins from the roots of pokeweed (Phytolacca americana). Acta Crystallographica Section D. 2004, 60(4): 665-673.