Pisum sativum Lectin (PSL) Production Services at CD BioGlyco

Plant Lectins have significant applications in the field of biology. CD BioGlyco is well established in the field of Lectin Production, and we offer our clients full process production of several types of lectins. Moreover, we have prepared many Related Products. PSL production services include but are not limited to the following.

• Extraction and purification

PSL is present in the roots of peas, we use a series of processes such as crushing, dissolving, adjusting pH, centrifugation, and filtration to obtain a solution containing lectin. Next, the solution is concentrated and lyophilized to obtain the crude product.

Then, the crude product is purified and collected by Gel Filtration Chromatography, Ion Exchange Chromatography, Affinity Chromatography, and other purification methods. Finally, we get the high-purity PSL.

• Characterization
• Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis method to determine the purity as well as the molecular weight of PSL.
• Using Lowry's method, we have determined the protein content of PSL.
• Use of the phenol-sulfuric acid method to determine the neutral sugar content of PSL.
• Using atomic absorption spectrometry, to determine the metal ion content of PSL.
• Using the hemagglutination assay and semi-antigen inhibition assay, the hemagglutination activity and sugar specificity are determined, respectively.

Fig.1 Procedure for the preparation of PSL. (CD BioGlyco)

Publication

Applications

• PSL is used to isolate cells, separate glycoproteins, and glycopeptides, and differentiate normal cells from virus-transformed cells.
• PSL is used to recognize glycoproteins, glycopeptides, and carbohydrates in biofilms and as a symbiotic medium for plants and microorganisms.
• PSL lectin is used to research the biological properties of cells and the mechanism of action of glycoconjugates.

Advantages

• We provide custom-produced solutions for our clients based on their research needs.
• Our service includes the whole process of extraction, purification, and property identification to save clients time.
• Our lectin products have high purity and good stability.

CD BioGlyco's service team has the world's leading lectin production technology to provide comprehensive lectin production services to our clients. Each production program we develop for our clients is subjected to rigorous quality testing to ensure client satisfaction. Please feel free to contact us if you are interested in our services and have any questions.

Reference:

1. Ng, T.B.; et al. Purification and characterization of a lectin from green split peas (Pisum sativum). Applied Biochemistry and Biotechnology. 2015, 177(6): 1374-1385.