High-quality Lectin Production Services at CD BioGlyco

Lectins are sugar-binding proteins of non-immune origin that agglutinate cells and precipitate glycoconjugates. Currently, lectins play an important role in the biological sciences, and CD BioGlyco provides Plant Lectins, Animal Lectins, and other types of Lectins Production Services. Among the plant lectins, the Polygonatum multiflorum (P. multiflorum) lectin production services are listed below.

• Extraction and purification services

We prepare the crude product of P. multiflorum lectin by pulverizing the roots of P. multiflorum, adding the extract to dissolve it, adjusting the pH, and going through the processes of precipitation, salting out, and so on.

Next, the crude product is dissolved with buffer and purified by thin layer analysis, silica gel column chromatography, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis to finally obtain high-purity P. multiflorum lectin.

• Performance testing services
  • Molecular weight analysis
    Use a combination of Ion Exchange Chromatography and SDS-PAGE to test the molecular weight of P. multiflorum lectins.
  • Protein identification
    Utilize SDS-PAGE electrophoresis and mass spectrometry techniques to perform protein identification.
  • Agglutination activity test
    P. multiflorum lectin and different erythrocyte suspensions are added to a hemocoagulation plate and using a microscope to observe the hemagglutinating activity of the lectin.
  • Sugar binding capacity assay
    Dissolve P. multiflorum lectin with buffer, add different sugar solutions to it, and assay the hemagglutination activity using the hemagglutination method. The sugar-specific binding capacity of the lectin is analyzed by comparing the hemagglutination results of different samples.
  • Stability test
    Thermal stability: To test the activity of P. multiflorum lectin at different temperatures.
    Acid-base stability: Dissolve it using buffers of different pH and test its activity separately.

Fig.1 Test and characterization services of P. multiflorum lectin. (CD BioGlyco)

Applications

• P. multiflorum lectin binds specifically to glycoconjugates, and it is used in research on signal transduction, immune response, plant defense, etc.
• In the medical field, P. multiflorum lectin is used in the development of anti-tumor drugs.
• P. multiflorum lectin is used to develop glycan-specific lectin probes to detect glycan structures.

Advantages

• Our lectin products are characterized with high purity and stability.
• Our production technology is suitable for high volume production of lectins.
• Our R&D team has the latest production technology and provides custom production solutions for our clients.

CD BioGlyco is dedicated to high-quality different types of lectins production services. Through precise synthesis processes and advanced technologies, we selectively achieve high-purity lectin production and provide tailor-made solutions to our clients. Please feel free to contact us for more details if you are interested in our services.

Reference:

1. Teka, T.; et al. Polygonum multiflorum: recent updates on newly isolated compounds, potential hepatotoxic compounds and their mechanisms. Journal of Ethnopharmacology. 2021, 271: 113864.