Protease from Streptomyces griseus

Synonym: Actinase E, Pronase E

MDL: MFCD00132092
CAS Number: 9036-06-0

Product Size

100 mg; 1 g; 5 g

Price

Datasheet

MSDS

Properties

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Form

powder

Purity

Type XIV, ≥3.5 units/mg solid, powder

Applications

Protease from Streptomyces griseus has been used to: • digest nucleus pulposus (NP) tissue • for deproteinization of starches • for digestion of brain slices for cell dissociation Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Streptomyces griseus has been used in crystallographic and kinetic investigations of the covalent complex formed by tetrapeptide aldehydes and serine proteases. Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA. The enzyme has been used for the digestion and analysis of antithrombin-heparin complexes. It has also been used for the isolation of enzyme-resistant starch. This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.

Safety Information

Personal Protective Equipment

Eyeshields, Gloves, type N95 (US)

WGK Germany

WGK 2

Hazard Statements

H315 - H319 - H334 - H335

Publications

Publications (0)

Publications

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