Recombinant Bacillus subtilis cellulase 5A produced by Escherichia coli, possesses broad exo-1,3/1,6-β-glucanase specificity and endo-β-1,4-glucanase activity.
Expression System
Escherichia coli
Species
Bacillus subtilis
Concentration
1 mg/mL
Form
Liquid
Purity
≥90%, determined by SDS-PAGE.
Activity
Cellulases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacillus subtilis cellulase 5A can be used in research for textile, animal food, pharmaceutical, detergent, and paper processing industries. It has the ability to hydrolyze 1,3-1,4-β-glucans, cellulosic carbohydrates, and lichenan.
Publications
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Publications
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