Recombinant Cellvibrio japonicus oligosaccharide 4-α-D-glucosyltransferase 31A produced by Escherichia coli, facilitates the transfer of the non-reducing terminal α-D-glucose residue from a 1,4-α-D-glucan to the 4-position of a free glucose or a glucosyl residue at the non-reducing terminus of a 1,4-α-D-glucan.
Expression System
Escherichia coli
Species
Cellvibrio japonicus
Concentration
1 mg/mL
Form
Liquid
Purity
≥90%, determined by SDS-PAGE.
Activity
Other Activities
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Cellvibrio japonicus oligosaccharide 4-α-D-glucosyltransferase 31A can be used in the synthesis of complex carbohydrates and glycoconjugates. It has the ability to hydrolyze maltopentaose, maltose, maltotetraose, and maltotriose.
Publications
Publications (0)
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Publications
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