Recombinant Geobacillus stearothermophilus α-glucuronidase 67A produced by Escherichia coli, breaks down the α-1,2 glycosidic bond between D-glucuronic acid or 4-O-methyl-D-glucuronic acid and terminal non-reducing D-xylose residues found in xylooligosaccharides (aldo-uronic acids) and xylan.
Expression System
Escherichia coli
Species
Geobacillus stearothermophilus
Concentration
1 mg/mL
Form
Liquid
Purity
≥90%, determined by SDS-PAGE.
Activity
Glucuronidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Geobacillus stearothermophilus α-glucuronidase 67A can be used in the paper pulp industry and preparation of xylans for pharmaceutical and cosmetic formulations. It has the ability to hydrolyze glucuronic acid.
Publications
Publications (0)
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Publications
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