Properties
Description
Recombinant Sphingomonas sp. oligoalginate lyase 15A1 produced by Escherichia coli, has the E.C. number 4.2.2.-. and specifically acts on alginate produced in Escherichia coli.
Expression System
Escherichia coli
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Sphingomonas sp. oligoalginate lyase 15A1 can be used in the production of bioactive oligosaccharides and participates in the eliminative cleavage of unsaturated oligoalginates.
