Biomedical Applications of Selective Exogenous (exo)-enzymatic Labeling (SEEL)

SEEL is a method applied to cell surface metabolic glycoengineering (MGE) for alteration and modification of cell surface glycan modification. With precise modulation of cell surface glycan structure, SEEL technology has vast potential for application. In the field of biomedicine, SEEL technology has been applied to drug design and vaccine development. In drug design, it is used to precisely regulate the glycan modification of drug molecules to improve drug targeting and delivery. SEEL technology provides various methods and strategies to enhance vaccine efficacy and safety. It allows researchers to modulate the structure of cell-surface glycans to alter the immunogenicity and immunization effect of vaccine antigens, and it can be used to construct specific vaccine delivery systems. These systems include nanoparticles, carriers, or nanofibers designed by modulating the sugar matrix structure to facilitate antigen uptake and delivery to the immune system, thereby improving vaccine efficacy.

Fig.1 Cell-surface engineering using an exogenously administered bi-functional CMP-Neu5Ac derivative and recombinant ST6GAL1. (Capicciotti, et al., 2017)

SEEL-based Cell Surface MGE Service at CD BioGlyco

CD BioGlyco is an outstanding biotechnology company specializing in research and development in the field of glycobiology. We utilize the advanced Glycoengineering Platform to provide clients with the most comprehensive SEEL-based Cell Surface MGE service. The details of our services are as follows:

• Firstly, we clone the exogenous gene encoding the target enzyme into an appropriate expression vector for expression in cells. The expression vector was then introduced into the target cells.
• Subsequently, we expressed exogenous enzymes in cells and used specific selective media to screen for enzymatically active cells. Then we add a glycan substrate to the cells to pass them through the surface of the cells. The exogenous enzyme will catalyze a chemical reaction at a specific site of the sugar substrate, such as the addition or deletion of a specific sugar moiety.
• Finally, we analyze the sugar modifications on the cell surface by various analytical methods.

Fig.2 The workflow of SEEL-based cell surface MGE. (CD BioGlyco)

Applications

• SEEL technology enables the synthesis of functional glycoconjugate probes targeting specific disease markers for early detection and monitoring of disease.
• SEEL technology is used to synthesize cells with specific sugar chain structures for tissue engineering studies.
• SEEL technology is used to synthesize tumor cell surface markers with specific sugar chain structures. These markers can be used as targets to develop targeted drugs or targeted immunotherapy.

Advantages of Us

• We change the structure of the substrate amidate by introducing different substrates, which extend the diversity of the glycan chains of your samples to a greater extent.
• SEEL technology allows control over the production of cell surface glycan chains by increasing or decreasing the supply of substrate, to better match your goals.
• SEEL technology can be applied to a wide range of cell types, as well as engineered with a choice of different substrate amide acids, allowing for flexible and customized design.

CD BioGlyco brings together the latest technologies and methodologies to provide clients with the SEEL-based cell surface MGE service. It serves as an important tool to help clients explore in greater depth the mechanisms and roles of cell surface glycan and medical issues. If you need the services mentioned above, please feel free to contact us immediately.