Tumor-Associated KH-1 Antigen Production Service
Tumor-Associated KH-1 Antigen Production Service at CD BioGlyco
Tumor-associated KH-1 antigen is highly expressed in many tumor types and is easily recognized and attacked by immune cells on the surface of tumor cells. Therefore, the KH-1 antigen as a vaccine candidate stimulates the immune system and generates specific T-cell and B-cell immune responses, thus enhancing the ability of the human immune system to recognize and attack tumors. There have been many studies on anti-tumor vaccines based on tumor-associated KH-1 antigen, and some research progress has been achieved. In response to the growing demand for Tumor-Associated Carbohydrate Vaccine Development, CD BioGlyco has developed a versatile Glyco™ Vaccine Development Platform that enables us to offer a comprehensive Tumor-Associated Glycolipid Antigen Production Service, including our professional tumor-associated KH-1 antigen production service.
- Firstly, we synthesize the backbone part of the KH-1 antigen by chemical synthesis or enzymatic synthesis. This backbone consists of specific sugar residues, such as L-rhamnose, fucose, N-acetylglucosamine, etc.
- Subsequently, we perform glycosylation on the synthesized backbone, grafting additional sugar residues onto the backbone by synthesizing activated sugar monomers and reacting with already existing sugar chains.
- Finally, we perform appropriate protecting group removal and modification of the synthesized sugar chains. The synthesized products are purified and analyzed after obtaining the target glycan chain structure.
KH-1 antigen is an important anti-tumor vaccine candidate. The synthesized KH-1 antigen can be used for vaccine development, preparation, and clinical trials. We offer subsequent anti-tumor vaccine development services based on the produced KH-1 antigen.
Fig.1 Flowchart for the synthesis of tumor-associated KH-1 antigen. (CD BioGlyco)
Applications
- The KH-1 antigen is highly expressed in a wide range of tumor cells and can be used as a tumor-specific marker for the development and validation of tumor assays.
- The KH-1 antigen can be used to screen and evaluate the efficacy and safety of drug candidates.
Publication
Paper title: Total synthesis of tumor-associated KH-1 antigen core nonasaccharide via photo-induced glycosylation.
Journal: Organic Chemistry Frontiers
Abstract: The main focus of this paper is the total synthesis of the tumor-associated KH-1 antigen core nine sugars using photoinduced glycosylation. This study provides new methods and strategies for the total synthesis of KH-1 antigen and lays the foundation for further understanding of KH-1 antigen and its application to tumor research.
Method: The main method of this paper is photo-induced glycosylation. The authors activated the photosensitizer by using ultraviolet light as an excitation light source and subsequently added alkaline reagents and other controlled reaction conditions to gradually construct the core non-saccharide structure of KH-1 antigen based on nine sugar units. The photo-induced glycosylation reaction is highly efficient, selective, and controllable, which allows the synthesis of complex sugar molecules while avoiding unnecessary protecting groups.
Frequently Asked Question
- What is the glycan structure of the tumor-associated KH-1 antigen?
The composition of tumor-associated KH-1 antigen consists of a protein core and sugar moieties attached to it. These sugar chains are usually formed by joining monosaccharide molecules in a specific order and linkage through specific enzyme-catalyzed reactions. Common sugar molecules include glucose, galactose, mannose, and N-acetylgalactosamine.
Fig.2 Structure of the KH-1 antigen. (Li & Guo, 2017)
- How to detect synthesized KH-1 antigen?
The synthesized KH-1 antigen can be detected by a variety of methods. Immunological tests include enzyme-linked immunosorbent assay (ELISA), immunofluorescence staining, and immunohistochemistry. Mass spectrometry and nuclear magnetic resonance spectroscopy can be used to directly determine information such as molecular weight and structure of KH-1 antigen. In practical applications, a combination of methods is usually required for comprehensive detection and analysis of synthesized KH-1 antigens.
CD BioGlyco produces high-quality tumor-associated KH-1 antigens to assist you in minimizing variables in your research and obtaining more precise results. Our goal is to provide you with a reliable tool that streamlines your research process in the field of tumor-associated studies. If you would like more information about our services, please feel free to contact us.
References
- Li, Q.; Guo, Z. Synthesis of the cancer-associated KH-1 antigen by block assembly of its backbone structure followed by one-step grafting of three fucose residues. Organic letters. 2017, 19(24): 6558-6561.
- Li, B.H.; et al. Total synthesis of tumor-associated KH-1 antigen core nonasaccharide via photo-induced glycosylation. Organic Chemistry Frontiers. 2020, 7(10): 1255-1259.
