Abrus precatorius lectin (APP) originates from the ground elder plant (Aegopodium podagraria). This protein exhibits specific carbohydrate-binding traits, particularly binding to select sugar structures found on glycoproteins and glycolipids. It displays a marked affinity for N-acetylglucosamine (GlcNAc) residues. At CD BioGlyco, our range of specialized services includes the provision of Lectin Production Services, including Natural Lectin Production Service, Recombinant Lectin Production Service, and Lectin Purification Service.

APP Extraction Service at CD BioGlyco

Rhizomes undergo homogenization using a mixer. The homogenate is then subjected to centrifugation. The resultant supernatant is separated and the coagulating material is precipitated through a subsequent centrifugation step. Following this, the supernatant is decanted and left overnight. Upon thawing, the coagulated part is precipitated by centrifugation, and the supernatant is brought to a relative saturation level. Protein precipitation is facilitated overnight and subsequently removed by centrifugation. The remaining supernatant allows the protein to precipitate, following which the protein fraction is collected via centrifugation and dissolved in PBS. Any insoluble components are centrifuged out, leaving behind a clarified solution that is then stored.

Fig.1 Process of APP extraction. (CD BioGlyco)

APP Purification Service at CD BioGlyco

• Ion-exchange Chromatography: Following the affinity purification process, APP undergoes subsequent purification on hydroxylapatite. The fractions containing the lectin desorbed from the affinity column are applied to a hydroxylapatite column that has been equilibrated with H₂O.
• Affinity Chromatography: Utilizing affinity chromatography, the ground-elder rhizome extract passes through a cross-linked agarose column containing immobilized erythrocyte membrane proteins, previously equilibrated with PBS. The unbound protein content is washed off by eluting the column with PBS.

APP Products at CD BioGlyco

We offer a range of APP products, including unconjugated APP and APP conjugated with different groups such as agarose conjugate, alkaline phosphatase conjugate, biotin conjugate, colloidal gold conjugate, ferritin conjugate, fluorescein isothiocyanate (FITC) conjugate, peroxidase conjugate, and rhodamine conjugate.

Applications of APP

• APP is used in laboratories for its ability to selectively bind to certain glycoconjugates on cell surfaces. This property makes it valuable in studying cellular interactions, cell signaling pathways, and various biological processes involving glycan-mediated recognition.
• APP is used in identifying and characterizing glycosylation patterns and alterations on cell surfaces or in tissues.
• The affinity of APP for specific glycan moieties is used for the purification of glycoproteins in biochemical and biotechnological applications.

Advantages of Us

• We possess specialized knowledge and techniques to isolate and purify APP with high purity.
• We adhere to rigorous quality control measures to meet the specific requirements of researchers and industries.
• We utilize advanced chromatography and purification methods to guarantee high specificity and purity of the APP.

CD BioGlyco provides professional Plant Lectin Production Services, especially APP production service. With rich experience and advanced technology, we ensure high-quality lectins are extracted and purified from plants. Customized solutions, research, and development are our strengths. Please do not hesitate to contact us for more details if you are interested in our APP production services.