Chitosan-Silver Nanoparticle Production Service
Overview of Chitosan-Silver Nanoparticles
Chitosan-silver nanoparticles (Ch-AgNPs) are hybrid nanostructures where silver nanoparticles are stabilized and functionalized by chitosan, a biodegradable and biocompatible polysaccharide. The Ch-AgNPs production service at CD BioGlyco offers advanced, eco-friendly solutions for synthesizing high-quality Ch-AgNPs. Chitosan acts as both a reducing and stabilizing agent, preventing aggregation and enhancing stability while maintaining biocompatibility. Based on our techniques like chemical reduction and green synthesis, our service ensures the high-quality and custom-amount production of nanoparticles.
Our GlycoNano™ Platform offers advanced Glyconanoparticle Development Services and several Techniques for Glyconanoparticle Development. We use methods such as chemical reduction, green synthesis, and sonication. These techniques are integrated into the development process to deliver stable, scalable, and highly effective glyconanoparticles for research and industrial use.
The production of Ch-AgNPs consists of two main steps: first, producing chitosan spheres (Chi-spheres), followed by incorporating silver nanoparticles to form composite structures (AgNPs-Chi-Spheres).
Stage 1: Synthesis of Chi-Spheres
Preparation of chitosan solution
Formation of spheres
Centrifugation
Washing
Drying
Stage 2: Synthesis of AgNPs-Chi-Spheres
Preparation of chitosan solution
Integration of silver
Formation of composite spheres
Centrifugation and washing
Drying
Glyconanoparticle Characterization Service for Ch-AgNPs
At CD BioGlyco, we offer comprehensive analytical services to ensure the quality and functionality of Ch-AgNPs. Our capabilities cover a wide range of physical, chemical, and biological analyses, enabling precise evaluation of nanoparticle size, structure, stability, and bioactivity.
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Physical Analysis |
Chemical Analysis | |
Biological Analysis |
Transmission electron microscopy (TEM) provides high-resolution images to observe the shape, size, and distribution of Ch-AgNPs.
Scanning electron microscopy (SEM) examines the surface morphology of nanoparticles, offering insights into particle uniformity and aggregation.
Dynamic light scattering (DLS) measures particle size distribution and hydrodynamic diameter, evaluating colloidal stability.
This technique evaluates the electrostatic stability of Ch-AgNPs in dispersion.
Fourier-transform infrared spectroscopy (FTIR) identifies functional groups and chemical bonds within Ch-AgNPs.
X-ray diffraction (XRD) determines the crystalline structure of the silver nanoparticles.
Ultraviolet-visible spectroscopy (UV-Vis) analyzes the optical properties of Ch-AgNPs by detecting surface plasmon resonance (SPR) peaks.
Energy-dispersive X-ray spectroscopy (EDX) confirms the elemental composition of Ch-AgNPs.
These assays evaluate the ability of Ch-AgNPs to inhibit bacterial and fungal growth.
Cytotoxicity assays assess the biocompatibility of Ch-AgNPs with human cells.
Applications
- Ch-AgNPs can be used in medical devices, food packaging, and surface treatments to inhibit bacterial and fungal growth.
- These nanoparticles aid in tissue repair and infection prevention, making them highly suitable for use in wound care and skin therapies.
- Ch-AgNPs effectively remove microbial contaminants in water treatment systems.
- Chitosan's biocompatibility and silver's antimicrobial effects combine to enable targeted drug delivery systems.
Advantages
- Ch-AgNPs combine the antimicrobial properties of silver with the biocompatibility of chitosan.
- Ch-AgNPs utilize non-toxic reagents and processes.
- Chitosan prevents nanoparticle aggregation and ensures long-term Ch-AgNPs are suitable for a wide range of applications, including biomedical and industrial fields.
Frequently Asked Questions
- What production scales are supported?
We support both small-scale research needs and large-scale industrial production. Our facilities enable scalable production from milligrams to grams while maintaining consistent quality. -
How are Ch-AgNPs evaluated for quality?
We use advanced analytical techniques like TEM, SEM, DLS, FTIR, and antimicrobial activity assays. These methods ensure precise characterization and validation of nanoparticle properties.
Publication
Technology: Chemical reduction, Chitosan stabilization, UV-Vis spectroscopy, FTIR spectroscopy
Journal: Polymers
IF: 4.7
Published: 2021
Results: This research investigated the production of AgNPs-Chi-Spheres, utilizing chitosan as a stabilizer and NaOH as a reducing agent, with NaOH concentrations varying between 20% and 50%. The resulting nanoparticles were characterized using various techniques, including UV-Vis spectroscopy, which confirmed surface plasmon resonance at 410 nm; FTIR and XRD analyses, which identified functional groups and crystalline structure; and SEM-EDX, which revealed smooth, spherical, and nonporous particles, with the smallest size of 46.91 nm at 20% NaOH. Zetasizer nano analysis showed good colloidal stability with high zeta potential values. At the same time, antimicrobial tests demonstrated broad-spectrum efficacy, with the highest inhibition zones observed at 50% NaOH against S. aureus (19.5 mm), E. coli (18.56 mm), and C. albicans (12.25 mm). The findings highlight AgNPs-Chi-Spheres as promising antimicrobial agents with stable and tunable properties.
Fig.1 (A) Chitosan (chi) and optimization product of synthesizing chitosan-spheres (chi-spheres) using (B) 20%; (C) 30%; (D) 40%; (E) 50% NaOH. (Mirda, et al., 2021)
At CD BioGlyco, our monosaccharide-gold nanoparticle production service provides advanced, customizable solutions for producing high-quality gold nanoparticles functionalized with monosaccharides. Please contact us today to learn how our products can help drive your research forward.
Reference
- Mirda, E.; et al. Synthesis of chitosan-silver nanoparticle composite spheres and their antimicrobial activities. Polymers. 2021, 13(22): 3990.
