Properties
Description
Galactose dehydrogenase-galactose mutarotase from Escherichia coli is responsible for oxidizing galactose to galactonate. Galactose mutarotase is from Escherichia coli that converts beta-aldose to alpha-aldose on some carbohydrates, such as D-glucose, L-arabinose, and D-xylose.
Unit Definition
One unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ per minute at pH 8.6, at 25°C.
Applications
Galactose dehydrogenase-galactose mutarotase from Escherichia coli is applied to the rapid measure of D-galactose or L-arabinose in the L-Arabinose/D-Galactose assay kit (K-ARGA).
