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O-Glycosidase for core 1 (Endo-α and Sialidase mix)
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Cat. No.: X24-08-LY326

O-Glycosidase for core 1 (Endo-α and Sialidase mix)

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Synonym: O-Glycosidase

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Product Size
1 Vial
Price
Datasheet
Datasheet
MSDS
MSDS
Properties
Description
O-Glycosidase for core 1 (endo-α and Sialidase mix) is an enzyme mixture that contains endo-α-N-acetylgalactosaminidase and sialidase.
Molecular Weight
Endo-α (approx. 210 kDa), Sialidase (approx. 886652 kDa)
Unit Definition
One unit of the endo-α will produce 1 μmol of Galβ1-3GalNAc from Galβ1-3GalNAc α-pNP per minute at pH 5.0 at 37°C. One unit of the sialidase will produce produce 1 μmol of N-acetylneuraminic acid (Neu5Ac) from Neu5Ac-α-4MU per minute at pH 5.0 at 37°C.
Reaction Conditions
50 mM Sodium acetate, pH 5.0
Detection Method
O-Glycosidase for core 1 is a mixture of Endo-α and Sialidase. Where Endo-α and Sialidase are detected separately. The product at the end of the endo-α reaction is measured by UV spectrometer. The product released by sialidase is measured by HPLC.
Sensitivity
Heat sensitive
Concentration
Endo-α 0.1 U per vial (1 U/mL); Sialidase 1.0 U per vial (10 U/mL)
Form
Colorless to almost colorless clear liquid
Titer
Endo-α (≥0.1 unit/vial), Sialidase (≥1.0 unit/vial)
Buffer
20 mM Sodium phosphate, 150 mM NaCl buffer, pH 7.4
Preservative
None
Stabilizer
None
pH
5.5
Applications
O-Glycosidase for core 1 (endo-α and sialidase mix) can be used for structural and functional analysis of glycan chains and to study the synthesis and modification of glycoproteins and glycolipids.
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Lacto-N-biose (Cat#: XCO0089Q) 2'-Fucosyllactose (Cat#: XCO0091Q) 3-Fucosyllactose (Cat#: XCO0092Q) Lactodifucotetraose (Cat#: XCO0093Q) Lacto-N-triose I (Cat#: XCO0094Q) 3'-Sialyllactose sodium salt (Cat#: XCO0096Q) 6'-Sialyllactose sodium salt (Cat#: XCO0098Q) 3'-Sialyl-3-fucosyllactose (Cat#: XCO0100Q)
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Heparin amine, MW 27 kDa (Cat#: X22-09-ZQ478) FITC-heparin, MW 27 kDa (Cat#: X22-09-ZQ480) TRITC-heparin, MW 27 kDa (Cat#: X22-09-ZQ481) Biotin-heparin-FITC, MW 18 kDa (Cat#: X22-09-ZQ482) Chondroitin sulfate (dp4) (Cat#: X22-11-ZQ598) Dermatan sulfate (dp12) (Cat#: X22-11-ZQ611) Heparin disaccharide I-A (Cat#: X22-11-ZQ662) Chondroitine sulfate (Cat#: X23-04-XQ1118)
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GalNAcβ(1-4)GlcNAcβ-Sp3-Biotin (Cat#: X22-12-ZQ005) GalNAcβ(1-4)GlcNAcβ-Sp3-PAA-Biotin (Cat#: X22-12-ZQ006) GalNAcβ(1-4)GlcNAcβ-Sp3-PAA-FITC (Cat#: X22-12-ZQ007) GalNAcβ(1-4)GlcNAcβ-Sp3-PAA (Cat#: X22-12-ZQ008) Glcβ(1-4)GalNAcα-Sp3-Biotin (Cat#: X22-12-ZQ037) Glcβ(1-4)GalNAcα-Sp3-PAA-Biotin (Cat#: X22-12-ZQ038) Glcβ(1-4)GalNAcα-Sp3-PAA-FITC (Cat#: X22-12-ZQ039) Glcβ(1-4)GalNAcα-Sp3-PAA (Cat#: X22-12-ZQ040)
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Dextran
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