Mannose-Magnetic Nanoparticle Production Service
Overview of Magnetic Glyconanoparticle
Comprised of metal elements, either in isolated or composite form, and their oxides, magnetic glyconanoparticles, particularly superparamagnetic magnetite, have emerged as a staple due to their superior biocompatibility and minimal toxicity. These iron oxide-based magnetic glyconanoparticles have increasingly captivated the scientific community, fostering advancements in understanding and application across various domains. Smaller-sized iron oxide magnetic glyconanoparticles are particularly favored in biological and biomedical applications. CD BioGlyco uses the advanced GlycoNano™ Platform, providing customized development and production of magnetic glyconanoparticles that integrate the controllable properties of magnetic materials with the biocompatibility and specificity of carbohydrate molecules. The surface chemistry of these magnetic glyconanoparticles is meticulously modulated to refine their physicochemical characteristics, enabling their deployment in diverse fields.
Empowering Precision Medicine with Mannose-Magnetic Nanotechnology
Among these, we perform a series of meticulously designed steps to create high-quality, mannose-coated maghemite nanoparticles.
Manufacture of Mannose-Magnetic Nanoparticle
- Synthesis of magnetic nanoparticles
We synthesize iron oxide nanoparticles by co-precipitating iron chloride precursors in deoxygenated water, followed by the addition of ammonium hydroxide. Wash the resulting black precipitate to adjust the pH to neutral.
- Sedimentation and acid treatment
Allow the solution to sediment under a magnetic field, discarding the supernatant. The black sediment is mixed with nitric acid and ferric nitrate, followed by dialysis against nitric acid.
- Coating with mannan
We mix the treated nanoparticles with mannan to coat the nanoparticles, adjusting the pH to neutral using ammonium hydroxide. This coating step is crucial for enhancing the nanoparticles' performance in various applications.
Characterization of Mannose-Magnetic Nanoparticle
After synthesis, characterization of the mannan-coated magnetic nanoparticle is conducted to determine key properties. We thoroughly assess the glyconanoparticles through two key components: the Glyconanoparticle Physicochemical Property Characterization Service, which evaluates the nanoparticles' size, charge, stability, and surface properties, and the Glyconanoparticle Molecule Interaction Analysis Service, which investigates the interactions between the glyconanoparticles and various biomolecules, ensuring optimal performance and compatibility in biomedical applications.
Workflow of Mannose-Magnetic Nanoparticle Manufacture
Publication
Technology: Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM)
Journal: 10.3390/catal9010007
IF: 10.6
Published: 2019
Results: In this study, the researchers optimized D-mannose-coated magnetic Fe3O4@OA@DP nanoparticles for immobilizing Escherichia coli cells that express recombinant glycerol dehydrogenase. TEM showed that the nanoparticles increased in size after mannose functionalization and effectively immobilized the E. coli cells. The optimal conditions for immobilization included a specific pH, cell concentration, and temperature, which maximized both yield and activity recovery. A certain buffer concentration was also found to be ideal for activity recovery. The immobilized cells performed better at higher pH and temperatures, retaining a high percentage of their initial enzyme activity after several hours of incubation, compared to a significant drop in free cells. Additionally, the immobilized cells maintained a substantial portion of their activity after multiple cycles, far outperforming free cells that retained only a minimal fraction of activity. The authors used the immobilized cells to convert glycerol to 1,3-dihydroxyacetone (DHA), achieving a significant improvement in production rate. This method eliminates the need for enzyme purification or expensive cofactors, reducing costs and making the immobilized cells highly promising for industrial applications, especially in DHA production.
Applications
- Mannose-magnetic nanoparticles act as macrophage-specific contrast agents for separating and identifying particular bacteria.
- The mannose-magnetic nanoparticle can be used for its ability to lower levels of IL-1β and TNF-α in the hippocampus to help manage inflammation.
- Mannose-magnetic nanoparticle plays a key role in blocking the surgery-upregulated HIF1-α/NF-κB pathway, essential for controlling inflammation and cellular stress.
Advantages
- Our synthesis process involves co-precipitation of iron chloride precursors in deoxygenated water and careful adjustment of pH, ensuring the production of high-quality iron oxide nanoparticles.
- We coat the nanoparticles with mannan, a process that enhances biocompatibility and specificity, making the nanoparticles particularly suitable for biological and biomedical applications.
- We provide customized development and production services, allowing clients to tailor the properties of the magnetic glyconanoparticles to specific needs and applications.
Frequently Asked Questions
- Why do we choose mannose for coupling with magnetic nanoparticles?
Mannose, a prevalent sugar found in numerous foods, plays a crucial role in the immune system as a component of the innate immune system's mannose-binding lectin (MBL). Its utility extends to serving as an economical backbone for synthesizing immunostimulatory and antitumor agents, enabling novel non-viral gene therapy approaches, and mediating natural killer cell function. - What technologies do we use for glyconanoparticle physicochemical property characterization service?
- Elemental Analyzer-based Glyconanoparticle Characterization Service
- HIC-based Glyconanoparticle Characterization Service
- XPS-based Glyconanoparticle Characterization Service
- FTIR-based Glyconanoparticle Characterization Service
- NMR-based Glyconanoparticle Characterization Service
- RS-based Glyconanoparticle Characterization Service
- SPR-based Glyconanoparticle Characterization Service
- TGA-based Glyconanoparticle Characterization Service
- Morphology-based Glyconanoparticle Characterization Service
- SANS-based Glyconanoparticle Characterization Service
CD BioGlyco offers high-quality and comprehensive Glyconanoparticle development Service via advanced techniques, ensuring superior biocompatibility and minimal toxicity. The modulated surface chemistry of our magnetic glyconanoparticles enables their deployment in a wide range of fields, from diagnostics research to targeted drug delivery research. If you want to obtain high-quality, mannose-coated magnetic nanoparticles, tailored to meet the specific needs of various scientific and industrial applications, contact us!
Reference
- Li, F.-L.; et al. Specific immobilization of Escherichia coli expressing recombinant glycerol dehydrogenase on mannose-functionalized magnetic nanoparticles. Catalysts. 2019, 9: 7.
