Properties
Description
Native PNGase F purified from Elizabethkingia meningoseptica is an N-glycan cleavage enzyme by cleaving between the innermost GlcNAc and Asn.
Unit Definition
One unit will catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at pH 7.5 at 37°C.
Source
Elizabethkingia meningoseptica
Applications
Native PNGase F purified from Elizabethkingia meningoseptica can be used to release intact N-linked glycans from glycopeptides and glycoprotein.