CD BioGlyco has extensive research experience in glycan study, our research team provides professional and custom NMR-based analysis services for our clients. 2D nuclear magnetic resonance (NMR) may be used to supplement 1D NMR analysis, which is very effective in simplifying the detection of molecular structures from a chemical mixture even without additional chromatographic separation.

What Are NMR-based Methods？

NMR is a powerful method for assessing biological mixtures' molecular structure, molecular interaction, molecular kinetics or dynamics, and composition. NMR-based glycan studies provide a significant advantage over chromatography and MS analysis for medium-sized polysaccharide molecules because NMR is more quantitative and reproducible and provides more comprehensive molecular structure information, such as ring chain linkage and conformation.

Fig.1 Structural Analysis of Glycans by NMR. (Lundborg & Widmalm, 2011)

NMR-based GSL Structure Analysis Service at CD BioGlyco

1D NMR (¹H and ¹³C NMR) is extensively employed in the characterization of polysaccharides. In both ¹H and ¹³C NMR spectra, the α- or β-configuration of the anomeric proton (H1) and anomeric carbon (C1) in sugar residue is visible. Because of the limitations of the ¹H NMR spectrum and the poor signals of the ¹³C NMR spectrum, 2D NMR technology is becoming more significant in the structural investigation of polysaccharides.

Fig.2 GSL NMR-based analysis strategies. (CD BioGlyco)

CD BioGlyco provides NMR-based GSL structure analysis services, and we offer our clients serval custom analysis proposals according to clients' requirements. We build an analysis platform that can program both 1D and 2D NMR-based analysis for GSL. Moreover, our research team has extensive experience in glycan chemistry and glycan biology.

Advantages of Us

• We provide high-quality analysis services for our clients worldwide.
• We offer our clients custom and suitable analysis proposals according to their needs.
• We establish an outstanding analytics platform with advanced instruments and extensive-experience scientists.

CD BioGlyco provides custom GSL structure analysis services for our clients, if you have any questions or require details information, please feel free to contact us.

References

1. Lundborg, M.; Widmalm, G. Structural analysis of glycans by NMR chemical shift prediction. Analytical Chemistry. 2011, 83(5): 1514–7.
2. Jamain, Z.; et al. Synthesis and determination of thermotropic liquid crystalline behavior of cinnamaldehyde-based molecules with two Schiff base linking units. Molecules. 2020, 25(17): 3780.
3. Knirel, Y.A.; et al. Structures and gene clusters of the O-specific polysaccharides of the lipopolysaccharides of Escherichia coli O69 and O146 containing glycolactilic acids: Ether conjugates of D-GlcNAc and D-GLC with (R)- and (s)-lactic acid Glycoconjugate Journal. 2016, 34(1): 71–84.
4. Lewis, I.A.; et al. Novel NMR and MS approaches to Metabolomics. Methods in Pharmacology and Toxicology. 2012, 199–230.
5. Ryzhkova, Y.E.; et al. One-pot solvent-involved synthesis of 5-o-substituted 5H-chromeno[2,3-beta] pyridines. Molecules. 2022, 28(1): 64.
6. Abreu, W.; et al. Tunicate heparan sulfate enriched in 2-sulfated β-glucuronic acid: Structure, anticoagulant activity, and inhibitory effect on the binding of human colon adenocarcinoma cells to immobilized P-selectin. Marine Drugs. 2019, 17(6): 351.