Peptide Loaded Glyconanoparticle Formulation Service
Overview
CD BioGlyco has a high-quality GlycoNano™ Platform to satisfy our clients in Glyconanoparticles Synthesis, Characterization, Preclinical Studies, and Formulation as a one-stop service. Our company offers many types of Biological Agent Loaded Glyconanoparticle Formulation services to meet clients' research needs in different areas. Our researchers aim to load specific peptide molecules onto glyconanoparticles to provide nano complexes with specific biological activities and functions. Such complexes have various applications in biomedicine, drug delivery, biosensing, and imaging.
Design Highly Stable Peptide Loaded Glyconanoparticles with Us
Our comprehensive peptide-loaded glyconanoparticle formulation service includes glyconanoparticle preparation, peptide molecule selection, loading, characterization, and optimization.
Glyconanoparticle Preparation
Glyconanoparticles are composed of carbohydrates and nanomaterials that are biocompatible and able to bind to peptides and stabilize their structures. We offer various methods for preparing glyconanoparticles, such as amide coupling, nucleophilic substitution, and CuAAC.
Peptide Synthesis
Our company mainly provides biosynthesis and chemical synthesis for peptide synthesis. We select the appropriate method for preparing peptides according to the specific situation and conditions of the peptide.
- Biosynthesis: For complex peptide chains, our researchers use biosynthetic methods. Biosynthetic methods mainly utilize cellular mechanisms and enzymes in organisms to synthesize peptides, including enzymatic digestion, fermentation, and genetic engineering.
- Chemical synthesis: Our company provides liquid-phase peptide synthesis (LPPS) and solid-phase peptide synthesis (SPPS) services.
Loading
We provide accurate peptide loading services such as glyconanoparticle surface activation and modification, incubation, cleaning, and real-time monitoring services. Our researchers load peptide molecules onto glyconanoparticles using specific chemical or physical methods based on carbohydrate and peptide properties.
Testing
- Appearance
- Encapsulation efficiency
- Polydispersity Index (PDI)
- pH
- Bioburden
- Encapsulation concentration
- Particle size
- Zeta potential
- Endotoxin
- Integrity of encapsulated peptides
Workflow
Applications
- Peptide-loaded glyconanoparticles can be used to improve peptide stability and provide a basis for chemo-immuno-combination therapy.
- Peptide-loaded glyconanoparticles can be used as a drug carrier for targeted delivery and controlled release of drugs.
- Peptide-loaded glyconanoparticles can be used for in vitro anti-tumor disease studies.
Advantages of Us
- Our company offers chemical synthesis methods with high accuracy and controllability for the preparation of peptides with different lengths and structures.
- Our company offers peptide-loaded glyconanoparticles with good biocompatibility and safety for biomedical applications.
- Our company offers peptide-loaded glyconanoparticles that remain stable during preparation and loading to avoid agglomeration or degradation.
Publication Data
Technology: Reversed-phase high-performance liquid chromatography (RP-HPLC), Liquid chromatography-mass spectrometry (LC-MS)
Journal: PLoS ONE
Published: 2018
IF: 3.752
Results: This literature focuses on the study of glyconanoparticles as a peptide drug delivery system and explores their ability to protect peptides from degradation by proteolytic enzymes in plasma. The researchers designed experiments to evaluate the protective effect of the glyconanoparticle delivery system on peptide drugs, including the preparation of glyconanoparticles, loading of peptide drugs, and evaluation of their stability in plasma. The experimental results showed that the glyconanoparticles delivery system significantly improved the stability of peptides in plasma and reduced their degradation by proteolytic enzymes.
Fig.1 Glycolliposome capture dynamic peptides and stability tests in plasma. (Lewicky, et al., 2018)
Frequently Asked Questions
- Why choose peptides as loading materials?
Peptides are a class of compounds consisting of three or more amino acid molecules that form peptide bonds through dehydration condensation. Peptides are involved in various physiological functions in the body as signaling molecules such as hormones, cell growth factors, neurotransmitters, etc., and play an important role in various life activities. The molecular weight of peptides is between that of small molecule chemical drugs and protein drugs, and they are characterized by high safety, controllable quality, high specificity, and high biological activity. We provide peptide synthesis and loading services of different lengths and structures according to clients' research needs. - What are the principles and advantages of LPPS of peptides?
LPPS utilizes a fully protected amino acid unit as the starting material and continuously adds amino acids to the solution to form peptide bonds with the previous amino acid, gradually growing the length of the polypeptide chain. This method is suitable for short peptide synthesis due to its fast synthesis speed, flexibility in amino acid selection, and tolerance to special sequences and chemical modifications.
CD BioGlyco has a professional research team to prepare nano-complexes with specific biological activities and functions through rational design and preparation processes, providing strong support for biomedicine, drug delivery, and other fields. Don't hesitate to contact us whenever you need assistance.
Reference
- Lewicky, J.D.; et al. Strengthening peptide-based drug activity with novel glyconanoparticle. PLoS ONE. 2018, 13(9): e0204472.
