Properties
Purity
lyophilized powder, recombinant, expressed in E. coli
Unit Definition
One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37 °C at pH 7.5 monitored by SDS-PAGE. One unit of PNGase F activity is equal to 1 IUB milliunit.
Applications
The highly purified material can be used for deglycosylation or for analytical applications in gels, solutions or blotting membranes. It can be removed by using its C-terminal 6x histidine fusion tag.