Properties
Description
PNGase F-II from Elizabethkingia meningosepticum is a recombinant endoglycosidase and catalyzes the cleavage of asparagine-linked oligosaccharides.
Unit Definition
One unit will deglycosylate 1 nanomole (15 μg) of denatured RNase B or 0.1 nanomole (4.5 μg) of horse radish peroxidase (HRP) in 2 h at 37°C.
Source
Elizabethkingia meningosepticum
Applications
PNGase F-II from Elizabethkingia meningosepticum can be used to cleave N-glycans linked with α1,6 or α1,3 core fucose.