Purposes of Radioactive Labeling

In 1935 the Hungarian radiochemist Georg de Hevesi was the first to use radioactive nucleotides to reveal the building blocks of rat metabolism. Since then, radioisotope labeling of nucleotides has been used in many medical research and clinical applications, such as the study of phage replication and clinical detection of cancer. They are enzymatically incorporated into DNA and RNA sequences for detection and analysis, such as generating information about gene integrity and copy number, detecting specific sequence and chromosomal aberrations, and more.

Radioactive Labeling Service at CD BioGlyco

With the mature Glyco™ Synthesis Platform, CD BioGlyco provides clients with high-quality nucleoside and nucleotide radionuclide labeling services. Based on detailed knowledge of specific aspects of radiochemistry and many years of extensive synthetic experience, we provide expert advice on route design for the synthesis of radioactive nucleosides and nucleotides and the optimal placement of radiolabels in the molecules. The radiolabeled compounds we synthesize are used in non-clinical research and drug development after rigorous quality testing.

Our labeled nucleosides and nucleotides include but are not limited to ribonucleosides (rNs), deoxyribonucleosides (dNs), ribonucleoside monophosphates (rNMPs), deoxyribose nucleoside monophosphates (dNMPs), ribonucleoside diphosphates (rNDPs), deoxyribonucleoside diphosphates (dNDPs), ribonucleoside triphosphates (rNTPs), deoxyribonucleoside triphosphates (dNTPs), and nucleoside building blocks.

Available radionuclides at CD BioGlyco include but are not limited to tritium (³H), carbon-14 (¹⁴C), phosphorus-32 (³²P), ³³P, and sulfur-35 (³⁵S).

All nucleosides and nucleotides we synthesize and label have their structure and purity determined by high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and mass spectrometry (MS), and deliver all analytical data for our global clients.

Fig.1 Radioactive labeling service. (CD BioGlyco)

Applications of Radioactive Labeling

• Detection of protein-nucleic acid interactions by fluorescence resonance energy transfer (FRET).
• Determination of relative expression of nucleic acids by microarray.
• Determination of specific sequences and chromosomal aberrations in cells or tissues using in situ hybridization.
• Generate information on DNA and RNA integrity and copy number.
• Measuring cell viability using labeled nucleotides.
• Study phage replication.
• Use in the research of disease detection.

Advantages of Us

• We combine radiolabeling expertise with excellent synthetic strategies, robust design, and efficient execution to deliver competitive products for our clients.
• Our experts will help you choose the best radiochemistry formulation for your needs.
• All products that we synthesize and label for our clients come with comprehensive analytical data packages.
• Our labeled nucleotides are used in a variety of applications including in situ allelic hybridization, microarray analysis, DNA sequencing, and Southern and Northern blotting.

CD BioGlyco provides different Modification Strategies of Nucleosides and Nucleotides to meet clients' project development goals. Please feel free to contact us for more information and a detailed quotation.