Properties
Description
Recombinant Endo F2 from Elizabethkingia meningoseptica is a commonly used endoglycosidase for cleavage of specific N-glycans.
Unit Definition
One milliunit will hydrolyze 1 nM of the dansylated porcine fibrinogen glycopeptide substrate DnsVGEN(CHO)R per minute at pH 4.5 at 37°C.
Source
Elizabethkingia meningoseptica
Applications
Recombinant Endo F2 from Elizabethkingia meningoseptica can be used to selectively release high mannose and double mannose complex N-linked oligosaccharides from glycopeptides/glycoproteins.